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Entamoeba histolytica

Entamoeba histolytica. Differentiation of amoebic and bacillary dysentery. Diagnosis. 1-Parasitic diagnosis a-Intestinal amoebiasis - stool -rectal ( exudate ) swab -material collected from the base of rectal ulcers b-Amoebic liver abscess -aspirated pus. A-Stool

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Entamoeba histolytica

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  1. Entamoebahistolytica

  2. Differentiationof amoebic and bacillary dysentery

  3. Diagnosis 1-Parasitic diagnosis a-Intestinal amoebiasis -stool -rectal (exudate) swab -material collected from the base of rectal ulcers b-Amoebic liver abscess -aspirated pus

  4. A-Stool 1)Microscopy -wet preparation (general stool examination) a-saline wet preparation (trophozoites) b-iodine wet preparation (cyst) -fixed preparation (permanent staining) -iron haematoxylin -trichrome 2) Concentration 3) Culture

  5. 2-Serodiagnosis 1)Antibody Detection -IHA -IFAT -ELISA 2) Antigen Detection 3-Molecular diagnosis PCR (polymerase chain reaction) 4-Radio-imaging diagnosis US, CT scan, MRI

  6. Epidemiology -Geographical distribution worldwide distribution 10 percent of the world's population 100,000 persons die -Source of transmission and infection Food and water cysts carriers -Infective form quadri-nucleated cyst -Susceptible population All ages

  7. Treatment 1-Luminal amoebicides diloxanidefluorate 2-Tissue amoebicides metronidazole (flagyl) {submucosa and liver tissue} chloroquine {liver tissue} tetracycline {submucosa}

  8. Blastocystishominis • -Morphological forms: vacuolated, amoeboid, granular and cyst forms. • -Distribution: worldwide. • -Habitat: large intestine. • -Infective stage: cyst form. • -Pathogenic role: is doubtful. • -Diagnostic specimen: stool.

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