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No stim.

MEEC. A. TGF- b (0.25 ng/ml). B. TGF- b (5 ng/ml). BAEC. - TGF- b. No stim. caALK1. caALK5. Smad7. Marker. H 2 O. LacZ. +TGF- b. 400 MOI F-Smad7. 1000 MOI LacZ. 1000 MOI F-Smad7. 400 MOI LacZ. 0.25. 0.25. PS1. 0.1. 0.5. 2.5. 0.1. 0.5. 2.5. 0.5. 1.5. 0.5. 1.5.

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No stim.

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  1. MEEC A. TGF-b(0.25 ng/ml) B. TGF-b(5 ng/ml) BAEC -TGF-b No stim. caALK1 caALK5 Smad7 Marker H2O LacZ +TGF-b 400 MOI F-Smad7 1000 MOI LacZ 1000 MOI F-Smad7 400 MOI LacZ 0.25 0.25 PS1 0.1 0.5 2.5 0.1 0.5 2.5 0.5 1.5 0.5 1.5 TGF-b (ng/ml): 0 5 0 5 0 1 0 1 Flag Smad7 actin PhosphoSmad1/5 C. Phospho-Smad2 MEEC BRE-luc 80 70 60 50 Luciferase activity (arbitrary units) 40 30 20 E. MEEC BRE-luc 10 MEEC CAGA12-luc 10 0 9 Mock 8 Smad7 7 6 Luciferase activity (arbitrary units) caALK1 5 4 MEEC 3 2 PGK-hygro Smad7-iRNAi D. 1 0 45 min 90 min 45 min 90 min TGF-b (min): - + + - + + 2h 3h 4h 2h 3h 4h Smad7-RNAi: 0 0 - - + - - + Smad7: Phospho Smad1/5 Phospho Smad2 actin

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