WP3 - Field experiments and sampling seeds for quality analysis.

1. WP3 - Field experiments and sampling seeds for quality analysis. AVEQ - Clermont-Ferrand, 19-21 September 2007

2. Partners: P1( Zofia Bulinska, Dorota Nowosielska IHAR Radzikow, Poland) P2 ( Rickard Jonsson, SW, Sweden) P5 ( Jean Koenig, INRA, France) P8 ( Luigi Cativelli, CRA-ISC, Italy) P9 ( Danela Murariu, SVGB, Romania) P10 (Nadja Antonova, IPGR Bulgaria) P11 (Külli Annamaa, Jogeva PBI, Estonia) SW PBI IHAR SVGB INRA CRA-ISC IPGR AVEQ - Clermont-Ferrand, 19-21 September 2007

3. The partners will perform field experiments for quality production and take over the following role: • Contribute to discussion of descriptors to observe in the field. • Establish 300 plots for accessions + 25-50 plots of standard • cultivars in two years. • Take observations in the field on agreed descriptors. • Take observations on harvested material (TGW, test weight, • hull content). • Distribute harvested seed to analytical labs. • Provide all observation results to the EADB. AVEQ - Clermont-Ferrand, 19-21 September 2007

4. Field design: The working collection (300 accessions each year) will be grown in field experiments in locations within seven European countries representing Nordic (Sweden and Estomia), Western European (France), Central European (Poland), South (Italy) and South East European (Bulgaria, Romania) growing conditions. Ten standard cultivars (two from each region) will be included in the experiment in five replications resulting in augmented designs. Plot size, sowing density, 3,9 m2, row distance 20cm, 6 rows, 1000 plants or 2 m2, row distance 20cm, 4 rows, 800 plants AVEQ - Clermont-Ferrand, 19-21 September 2007

5. Suggested descriptors, need to be discussed • Colour of lemma (1. White 2.Yellow 3.Grey 4. Red 5.Brown 6.Black) • Color of kernel (1. White 2.Yellow 3.Light Brown 5.Brown 6. Dark brown) • Days to heading and to maturity ( rating when first spikelet visible in • 50% of panicle) • Crop height: • 1 Very Short • 2 • 3 Short • 4 • 5 Medium • 6 • 7 Tall • 8 • 9 Very Tall AVEQ - Clermont-Ferrand, 19-21 September 2007

6. Suggested descriptors, need to be discussed - ctd • Lodging resistance and rating: • 1 Resistance very high - no lodging • 3 Resistance high - low lodging • 5 Intermediate • 7 Resistance low - high lodging • 9 Resistance very low - very high lodging • or • 1 Upright (all plants) • 3 Minor lodging • 5 Intermediate • 7 Lodged • 9 Extremely lodged ( all plants) AVEQ - Clermont-Ferrand, 19-21 September 2007

7. Suggested descriptors, need to be discussed - ctd • Panicle shape Unilateral, subunilateral, equilateral AVEQ - Clermont-Ferrand, 19-21 September 2007

8. Suggested descriptors, need to be discussed - ctd • Number of sterile spikelets • 1 Very Few • 2 • 3 Few • 4 • 5 Medium Number • 6 • 7 Many • 8 • 9 Very Many • Occurrence of diseases – (scale 1- 9) AVEQ - Clermont-Ferrand, 19-21 September 2007

9. Suggested descriptors, need to be discussed - ctd • Hull content • (partners with dehulling equipment, e.g. P3 50-60 g - husked seeds treated 2x30s with a lab threshing machine (SELECTA Type 1-AW-SRA): air flow 6 units, speed of rasp- bar cylinder 8. 200 dehusked kernels weighed ). • Thousand kernel weight • (200-kernel samples in triplicate). • Test weight (kg/hl) AVEQ - Clermont-Ferrand, 19-21 September 2007