2013 Korea - Ukraine Science Forum Electrodynamic Introscopy (EI) of Living Tissues Presentation:

1. 2013 Korea - Ukraine Science Forum Electrodynamic Introscopy (EI) of Living Tissues Presentation: Yuri F. Babich & Vitaly B. Maksymenko Faculty of Biomedical Engineering NTTU “KPI”

2. EI is an original type of instruments for medical imaging, which allowed firstly in the world* to visualize the Skin Electrical Landscape - initially in statics and later in multiparameter dynamics. healthy > The earlier EI recordings sick > Scheme of APs of ear lobe Electroimpedance landscape * 1st prize at the World Congress on Medical Physics and Biomedical Engineering, Nice, 1997

3. The today 6-channeled EI experimental setup: General view of the EI scan-head and an example of the EI experimental use. • Characteristics: • - The scanning is performed contactly using electromagnetic radiation of extremely low intensity; • - Simultaneous measurement of: spectral electroimpedances and electropotentials (2 kHz -1 MHz); • - Spatial resolution of ≤ 1 mm; • - Scan area – 32x64 mm. • Frame time – 8s. And here, with the dynamic imaging, it turned out that the APs are not always stationary objects…

4. In a row of lab-clinical trials the skin electrodynamics proved to be highly sensitive to exposure of various environmental factors: ● weak magnetic fields, ● non-thermal mm-EMF, ● mild pharmaceutical irritants, including allergy tests. ● hypoxia and hyperoxia etc.

5. The spectral characteristics enable to distinguish between extra-cellular and intra-cellular events Schematic indication of flow currents at various frequencies and magnitude of induced dipoles. < Particularly intriguing is the EI ability to in vivo assess the mitochondria state

6. Novel macroscopic phenomena of spatial electrical activity of the skin have been revealed, specifically: wave-like and pulsating structures with a velocity of in-vivo known waves of calcium signaling. Such visualization of metabolic and information processes can be used both: ● as an early diagnosics, ● as a real time biofeedback for various biomedical purposes, like, e.g. online estimation of influence of therapeutic or environmental factors.

7. Some illustrations Malignant melanoma and its environment to the eyes of EI. < Photo – the surrounding tissue looks quite healthy; Cellular level(a difference image): suspicious micro-regions, which came out in response to hypoxia; Subcellular level : the dark areas represent functional abnormalities (presumably mitochondrial ones).

8. Novel characteristics of living tissue in parameters of cell-cell (pixel-to-pixel) synchronization in norm and pathology: a) healthy tissue - normal homeostatic fluctuations around the equilibrium point; b) marked abnormalities (synchronized pathological process) on the melanoma border; c) similar process in a case of suspicious nevus.

9. Melanoma with satellite under influence of hypo- and hyperoxia satellite Difference images of the SEL in responses to hypo- and hyperoxia tests reveal noticeable distinctions both: in spatial manifestation and parametrically opposite dynamics. < Calculated map of synchronicity with regard to the induced dynamics of the satellite

10. Just a formal comparison with a Siemens analogue Matrix step = 1mm. 2048 electrodes Matrix step ≈ 6-10 mm, ≈ 80 electrodes An EI experimental sample Conventional electrode array forbreast imaging

11. Primary Areas of the EI Possible Implementation Oncology Gerontology Diagnostics, targeted therapy, biomedical research Drug discovery Tissue engineering Dermato- cosmetology Detection, therapy

12. Currently our goal is establishment of a proper collaboration on conclusive joint-R&D at the base of modern western laboratory and further implementation of the EI Thank you for your attention!