MICROBIOLOGY Practical Class

1. MICROBIOLOGYPractical Class FOUNDATION BLOCK (2018) Dr . Malak M. El-Hazmi

2. MICROBIOLOGY

3. Laboratory diagnosis of infectious diseases • Microscopic examination. • culture. • Serological tests (Ab). • Detection of Ag. • Molecular method .

4. Types of specimens

5. Bacteriology

6. Gram Stain G- bacilli

7. Bacterial cell wall

8. Bacterial Shapes and Arrangements

9. Gram Stain

10. GRAMPOSITIVEBACTERIA

11. GRAMPOSITIVECOCCI

12. GRAMPOSITIVECOCCI

13. GRAM NEGATIVE BACTERIA

14. Gram negative cocci (Diplococci ) e.gNeisseria • Gram negative bacilli • e.g E. coli • Salmonella

15. Gram positive cocci in chain Streptococci Gram positive cocci in clusters Staphylococci Rx cloxacillin Cephalosporin if MRSA vancomycin Penicillin Cephalosporin

16. A gram-stained smear of a CSF sample from a 3 year old child seen in the emergency department presenting with fever and neck stiffness. Gram-positive diplococci & pus cells Streptococcus pneumoniae

17. This is a bacterium isolated from a child with sore throat and tonsillitis . A: Describe the Gram stain Gram positive B: Describe the shape and arrangement of the bacteria Cocci in chains

18. Following is the Gram stained smear of an organism isolated from a wound infection. Describe what you see in the slide above. Gram-positive cocci in clusters What is the likely organism ? Staphylococcus aureus

19. Following is the Gram-stained smear of from urethra of a 25 –year old male complaining of urethral discharge Describe the Gram stain of the intracellular bacteria Gram negative Describe the shape of the bacteria cocci ( diplococci)

20. Describe the Gram stain of this organism: Gram negative Describe its shape bacilli ( rods )

21. Bacterial Culture Media

22. Bacterial Culture Media

23. Bacteria culturing 1-Inoculation 3-Incubation 2-streaking Laboratory Incubator

27. Identification of streptococci by hemolytic reaction Beta-hemolytic Streptococcus colonies Alpha-hemolytic Streptococcus colonies Gamma-hemolytic Streptococcus colonies

28. GRAM NEGATIVE BACTERIA

29. MacConkey's agar (Deferential medium)

30. Biochemical testings • To confirm • the identity • of bacteria. Antibiotic susceptibility testings

31. Automated instrument for identification and susceptibility testings

32. Blood agar This is a general culture medium used for culture of bacteria.

33. This is a blood agar growing beta hemolytic streptococci.

34. This culture was grown from a sputum specimen of a 60 year old man complaining of cough, fever and chest pain. α hemolytic streptococci on blood agar

36. VIROLOGY

37. Viral structure

38. Viral Classification

39. Viral Electron Micrographs

40. Herpes simplex virus -1 : Herpesviridae Enveloped virus Icosahedral capsid d.s DNA genome Loose envelope

41. These are electron micrographs of a virus Herpes virus Q1: Name this virus Enveloped virus , Icosahedral capsid, d.s DNA genome Q2: Describe its structure.

42. Adenovirus : Adenoviridae Nonenveloped virus Icosahedral capsid d.s DNA genome Only V with fiber

43. This is an electron micrograph of a virus Adenovirus Q1: Name this virus • Nonenveloped virus, with fiber • Icosahedral capsid & d.s DNA genome Q2: Describe its structure.

44. Rabies virus: Rhabdoviridae Enveloped virus Helical capsid s.s RNA genome Bullet shape

45. This is an electron micrograph of a virus Q1: Name this virus • Rabies virus Enveloped virus , Helical capsid & s.s RNA genome Q2: Describe its structure.

46. Influenza Viruses : Orthomyxoviridae Enveloped V & spikes Helical capsid Segmented s.s RNA Pleomorphic shape

47. This is an electron micrograph of a virus Q1: Name this virus Influenza Viruses Enveloped Virus with spikes , Helical capsid ,Segmented s.s RNA Q2: Describe its structure

48. parasitology

49. Classification of Parasites

50. Ascarislumbricoides (roundworm) Ascaris adult