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Mark Prescott Department of Biochemistry and Molecular Biology

Explore genetically encoded chromophores, crystallography, and biological applications in fluorescence research. Collaborative projects and future developments. Investigating dark acceptors and chromobodies for in vivo labeling.

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Mark Prescott Department of Biochemistry and Molecular Biology

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  1. Network in Fluorescence Applications in Biotechnology and Life Sciences Mark Prescott Department of Biochemistry and Molecular Biology

  2. Genetically encoded chromophores • Structure • Develop as useful tools • Biological applications

  3. Crystallography approach to 3-Dimensional Structure

  4. Crystals of some chromoproteins and fluorescent proteins Collaboration with Jamie Rossjohn, Monash

  5. EqFP611 DsRed Rtms5 • Collaboration with HcRed • Photo-chemist? Alternative chromophore configurations

  6. Rtms5 T-Sapphire Rtms5 Retrieving spectral space chromoproteins as ‘dark’ FRET acceptors for FLIM

  7. ‘Chrombodies’ In vivo labeling reagents for FRET/FLIM Grafted CDRs N C In collaboration with Andrew Bradbury – Biosciences Division, Los Alamos

  8. Mitochondrial membrane structure ATP synthase and cristae Probe and model arrangement in vivo using FRAP and FCS

  9. Some challenges • Isolate and characterise further genetically encoded fluorescent labels (far-red emissions). • Understand the post-translational alterations that lead to the optical properties of these proteins (eg: photo-conversion). • Develop genetically encoded chromoproteins for use as ‘dark’ acceptors in FLIM applications. • Develop libraries of ‘chromobodies’ to significantly extend possibilities labeling possibilities in vivo. • Investigate environment / liftetime relationships in vivo for FPs. • Improved sensitivity

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