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Here is what we look for in the histogram.

Here is what we look for in the histogram. We try to make sure we get the histogram to match up on top of each other and it in the 1E-4 to -5 range. The spike in the red channel is from our control probe that we use to line up the grid from extracting the probes. The whole image.

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Here is what we look for in the histogram.

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  1. Here is what we look for in the histogram. We try to make sure we get the histogram to match up on top of each other and it in the 1E-4 to -5 range The spike in the red channel is from our control probe that we use to line up the grid from extracting the probes.

  2. The whole image

  3. Close up of both cy3 and cy5 Control probes

  4. Cy3 only

  5. Cy5 only

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