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Topic : Microscope Aim : How do we use the compound microscope? Do Now :

Topic : Microscope Aim : How do we use the compound microscope? Do Now : How to use a microscope reading ISA (4 minutes) Take out yesterday’s microscope notes HW : 1. Finish the Microscope ISA 2. Castle Learning: Cell Respiration and Transport  Due Friday.

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Topic : Microscope Aim : How do we use the compound microscope? Do Now :

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  1. Topic: Microscope Aim: How do we use the compound microscope? Do Now: • How to use a microscope reading ISA (4 minutes) • Take out yesterday’s microscope notes HW: 1. Finish the Microscope ISA 2. Castle Learning: Cell Respiration and Transport  Due Friday

  2. Total magnification: Eyepiece x Objective

  3. 100x 400x

  4. 4x Power = 10x = 40x x

  5. 400x 10x 10x 40x 4x 100x 10x 40x 10x 1. Describe what occurred to the size of the image when switching from 4x to 10x to 40x objectives. The specimen APPEARS larger.

  6. Low Power High Power

  7. 400x 10x 10x 40x 4x 100x 10x 40x 10x 2. Does the field of view increase or decrease as you switch from the low power objective to the high power objective? Support your answer. Field of view decreases. You see less of the slide.

  8. 400x 10x 10x 40x 4x 100x 10x 40x 10x 3. Which objective should be used when searching for the specimen on the slide? Support your answer. Low power because it has a larger field of view.

  9. How do images appear? Image on slide BEFORE placing it on the stage to view How the image appears when viewing with the microscope e

  10. On the slide Seen through lens F

  11. What happens when you move the slide? Image moves in opposite direction

  12. How must you always carry a microscope? Always carry with 2 hands. One hand on arm and one hand under the base

  13. High power objective Low power objective e e • If you switch to high power when the image is positioned in the diagram above, how will the image appear under high power? • Identify a procedure that must be followed before switching to high power. It will not be in your field of view. Center your image.

  14. Low power objective High power objective

  15. Low power objective High power objective Center your image before switching to high power!!!!

  16. What two procedures must be done BEFORE switching to high power? - Focus your image - Center your image

  17. What happens to the amount of light in your field of view when switching to high power? The amount of light DECREASES. Adjust the diaphragm to make it brighter.

  18. What do you use to clean the microscope lenses? Only use LENS PAPER for cleaning

  19. Fine adjustment Let’s summarize…Identify the microscope part described. • Used to focus the specimen under high power. • Adjusts the amount of light reaching the specimen. • Platform slide is placed on. • Objective used to view a larger field of view needed to search for a specimen. • Used to focus specimen under low power • Structure that you look through. • Contains lenses. • Keeps slide in place. • Objective used to view a larger and more magnified view of the specimen. • Objective used to search for a specimen. Diaphragm Stage Low power objective Coarse adjustment Ocular Ocular and objectives Stage clips High power objective Scanning obj.

  20. Aerobic respiration C6H12O6 + 6O2 6CO2 + 6 H2O + X • Identify the process represented above. 2. Identify the raw materials. 3. Where does this process occur? 4. Identify the waste products. 5. Identify molecule X. 6. Describe the importance of this process. glucose and oxygen Mitochondria carbon dioxide and water Energy or ATP It provides energy that is needed for all cell activities.

  21. Why do we stain cells? Unstained cheek cells Stained cheek cells

  22. Cheek Cells Unstained Stained with Methylene Blue

  23. Skin Cells stained with Methylene Blue

  24. Onion Cells Unstained Stained with Lugol’s Iodine

  25. Why do we stain cells? To Make cell parts more visible

  26. Wet mount

  27. Place a drop of water on the slide

  28. 2. Place object on the slide.

  29. 3. Slowly lower the cover slip on an angle to prevent AIR BUBBLES.

  30. 4. Absorb excess water with a paper towel.

  31. Let’s summarize… • Explain one rule that must be followed when using the microscope. • What do we use to make cell parts more visible? • Which way will a cell appear to move when moving the microscope to the left and down? • What happens to the image of the specimen when viewed with a microscope?

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