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Supplemental digital content. YST-1. si Ctrl. si Bec-1. Beclin-1. b -actin. si Ctrl. si Bec-1. 0 30’ 60’ 90’. 0 30’ 60’ 90’. pERK42/44. ERK42/44. b -actin.
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Supplemental digital content YST-1 si Ctrl si Bec-1 Beclin-1 b-actin si Ctrl si Bec-1 0 30’ 60’ 90’ 0 30’ 60’ 90’ pERK42/44 ERK42/44 b-actin
Figure legendSupplemental digital contentYST-1 cells were transfected with negative control, beclin-1 siRNA as indicated. After 72 h of incubation, siRNA-transfected cells were incubated with imatinib (20 microM) from 30 to 90 minutes, and then total cell lysates were resolved by SDS-PAGE and immunoblotted with anti-beclin-1, anti-phospho-ERK1/2, anti-ERK1/2, or anti-b-actin antibodies.