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Introducing automated ELISA - ChemWell TM

Introducing automated ELISA - ChemWell TM. Geoffrey Anderson Unitech Scientific LLC Lakewood, CA Visit us: www.unitechscientific.com. ChemWell TM Wine Autoanalyzer. Manufactured by Awareness Technology Unitech Scientific - Exclusive Distributor.

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Introducing automated ELISA - ChemWell TM

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  1. Introducing automated ELISA - ChemWellTM Geoffrey Anderson Unitech Scientific LLC Lakewood, CA Visit us: www.unitechscientific.com

  2. ChemWellTMWineAutoanalyzer Manufactured by Awareness Technology Unitech Scientific - Exclusive Distributor

  3. First analyzer in the world to combine biochemistry and microplate EIA into one instrument. ChemWell uses microwells for all reactions, chemistry and EIA.

  4. A ChemWell Tour Optical system 2 syringe pumps Wash head Windows 95, 98 or XP Wash and rinse bottles reactions Reagents Samples DI water

  5. ChemWell Designed for labs doing: 100 - 1000 tests per day or 400 EIA per 8 hours Recommended workload

  6. ChemWellFeatures & Benefits • Easy to Use • Continuous Sample Loading • “Stat” sample priority • Low Operating Cost • Intuitive, Open Software • Proven, Reliable Technology

  7. Calibration Options • Cut-off (Semi Qualitative) • Multi-point Polynomial fit • Cubic Spline fit . . . and more Store Calibration Curves Wine Controls: Chart Levy Jennings

  8. ChemWell runs ELISA as well as Enzymatic reagents Unitech offers Histamine ELISA kits Botrytis and Aspergillus ELISA kits are in development So let’s take a quick review of the steps and principals of ELISA reagents.

  9. Immuno Complex:an Antigen with its matching Antibody Ag stands for Antigen Ag Ab stands for Antibody Ab An antigen and its antibody fit together like 3 dimensional puzzle pieces.

  10. Diagram of a simple ELISA with a positive sample (sample has Ag in it) • Mix the sample and diluent together in a microwell that is pre-coated with specific Ab • Incubate for x minutes until the Ag in the sample binds to the Ab on the well walls. Start with an Add the sample Incubate until Ab-coated well with some Ag in it complexed Ag Ab Ab Ab-Ag Ag Ab-Ag Ab Ab Ab Ab Ab Ab

  11. Conjugate is a specific Ab bound to an Enzyme. Wash out the Add a Incubate until remaining sample conjugate complexed Ab-E Ab-Ag-Ab-E Ab-Ag Ab-Ag Ab-E Ab Ab Ab Ab-Ag-Ab-E Ab-Ag Ab-Ag Ab-E Ab-E The conjugate has a second antibody to the same antigen, allowing you to make an antigen sandwich (with antibody bread). It also leaves an enzyme hanging off.

  12. Washing . . . removes the unbound conjugate, without removing bound antibodies. Wash out the Add a Substrate + Enzyme remaining conjugate substrate produces color S Ab-Ag-Ab-E-S Ab-Ag-Ab-E Ab-Ag-Ab-E Ab Ab Ab Ab-Ag-Ab-E-S Ab-Ag-Ab-E S Ab-Ag-Ab-E S S The more Enzyme available, the more color will develop. Many ELISA’s use a stopping solution to end the color generating step before reading.

  13. Reliable Results:Enzymatic and ELISA Precise pipetting Washes probe inside and out Liquid sensing Temperature controlled

  14. Easy to Maintain • Proven Reliability, over 500 sold • Self Diagnosis • Phone/email support by Unitech • Modular Design

  15. Economical to Use • Dramatically reduces LABOR • Laboratory • Administrative

  16. Warranty • 1-year parts & labor Warranty • Extended Warranty available • 6-Month Preventative Maintenance • Free replacement of warranteed parts • Other repairs if required • Loaner instrument within 72 hours

  17. Easy to own • Price start at $27,500 • Reagent rack cooling option Add $2000

  18. ChemWell Distributed by Unitech Scientific

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