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Real-time PCR automated solution for food pathogen testing. Jean-Philippe Tourniaire – International Product Manager. Lab Innovations, Birmingham, November 6 th 2013. iQ -Check Prep Solution goals. Straightened our global offer on iQ -Check Food pathogen testing
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Real-time PCR automated solution for food pathogen testing Jean-Philippe Tourniaire – International Product Manager Lab Innovations, Birmingham, November 6th 2013
iQ-Check Prep Solution goals • Straightened our global offer on iQ-Check Food pathogen testing • Provide technically advanced solutions to medium to high throughput laboratories • Provide confidence and peace of mind with a cost effective solution
iQ-Check Prep Solution Concept • Complete automation of the iQ-Check Easy protocols • From the enriched sample to the PCR plate ready • Two plates handled at the same time • Run of up to 4 different parameters on a plate • Compatible with all the iQ-Check Food kits • Optimal integrated solution with user-friendly interface: CFX Manager software IDE • Partnership with Hamilton http://www.hamiltonrobotics.com, a WW leader in automation
Automation Concept Automated
Deck description Rack 2 Non mobile Rack 3 mobile Rack 4 mobile Rack 1 mobile 1000 µL tips Agitator / incubator DW plate 2 for extraction 50 µL tips DW plate 2 for samples Gripper 1 2 3 4 5 6 7 8 Tubes for PCR mix Waste DW plate for dilutions (Transfer) PCR plate loading place Lysis bottles 2 3 DW plate 1 for extraction 1 4 PCR plates 1& 2 DW plate 1 for samples 1 2 3 4 1000 µL tips PCR reagents kits Water container
Technology • 4 channels Air displacement pipetting principle Tips pick-up with CO-RE Technology Type of tip recognition
Technology Liquid Level Detection: LDD Added value of the conductive tips: Liquid level sensing (aspiration / dispensing) for increased safety and confidence Monitored Air Displacement: MAD
iQ-Check Prep User Interface Two computers and software needed: • CFX ManagerTM Software IDE v2.0 : handles the CFX96 Deep WellTM Touch (and MiniOpticonTM) and allows the plate set-up for the iQ-Check Prep
iQ-Check Prep Software - User Interface • iQ-Check Prep Software v1.3: gets information from the CFX Manager IDE v2.0 and handles the protocols on the iQ-Check Prep instrument. The user is guided through the different steps to start the run : refill of consumables and reagents
Throughput Capacities / Workflow • Highest throughput configuration: • 2 x 94 samples / prep run : about 2 x 70 min • 2 hrs PCR • Process 6 plates in 8 hrs • Time duration of different protocols or steps • Complete run : 1h10 min • DNA already extracted: 30 min • 1/10 dilution: + 15 min • Lysis reagent dispensing: 8 min • PCR mix preparation and dispensing: 19 min • Cooling the Deep Well plate (DNA extract): 25 min
Throughput Capacities / Workflow Configuration 1: 1 iQ-Check Prep + 1 CFX96 Deep Well Touch IDE More than 250 samples tested in 8 hrs Configuration 2: 1 iQ-Check Prep + 2 CFX96 Deep Well Touch IDE More than 500 samples tested in 8 hrs
Absence of Cross Contamination One of the major concern for this project: tested many times with highly positive and negative samples • Perfectly clean results and deck!!!
Key Benefits • Easy to use • LIMS connection to import & export data • Improved throughput capacities • Improved traceability • Increased accuracy: less handling errors • Save technician handling time • Simple transposition of the manual protocol (not a black box) • Pipetting control • Limited maintenance (air displacement) • AFNOR and AOAC validated
Feedback From Users J. P., evaluator • “we really like the Prep station …. The advantages are that it virtually eliminates the potential for liquid handling and cross contamination errors. The operation of the Prep station is straightforward and the software is easily navigable providing the operator with prompts, diagrams and help/troubleshooting screens. The PCR detection software is also user-friendly and intuitive….” C.S., evaluator • Easy to use • Great and improved PCR results • Save minimum 1 hr technician time / day From competitor • “It’s hard to make it simple, and you did it!”
iQ-Check Prep Solution Thanks a lot for your attention!