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15d-PGJ 2 -. MMP-1. Actin. MMP-1. GAPDH. MCF-7. Supplementary 1. p<0.005. p<0.005. 12. 10. 8. Number of migrated cells (x10 3 ). 6. 4. 2. 0. + MMP-2 Ab. + MMP-1 Ab. + MMP-3 Ab. + MMP-7 Ab. + MMP-9 Ab. + MMP-11 Ab. + MMP-13 Ab. dilution buffer. dilution buffer.
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15d-PGJ2 - MMP-1 Actin MMP-1 GAPDH MCF-7 Supplementary 1
p<0.005 p<0.005 12 10 8 Number of migrated cells (x103) 6 4 2 0 + MMP-2 Ab + MMP-1 Ab + MMP-3 Ab + MMP-7 Ab + MMP-9 Ab + MMP-11 Ab + MMP-13 Ab dilution buffer dilution buffer + 15d-PGJ2 Supplementary 2
(B) (A) SnPP - - + - + +++ - 15d-PGJ2 MMP-1 GAPDH 15d-PGJ2 (C) 0 6 24 (h) 12 3 MMP-9 ZnPP - - + GAPDH - + +++ - 15d-PGJ2 CoPP MMP-1 (D) 0 3 6 12 24 (h) GAPDH MMP-9 Control 15d-PGJ2 (E) Scramble siRNA + - + - - + - + HO-1 siRNA MMP-9 GAPDH Supplementary 3