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Structure of MMP-iresGFPneo. Genome length 4294 nt, without transgene Capacity ~4,100 bp. LTR. LTR. Y. Transgene. Provirus. GFP. neo. IRES. SD. SA. Y. Transgene. m7 G-. -A n. RNA Genome. GFP. neo. IRES. SD. SA. Transgene. Spliced Message. m7 G-. -A n. GFP. neo. IRES.
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Structure of MMP-iresGFPneo Genome length 4294 nt, without transgeneCapacity ~4,100 bp LTR LTR Y Transgene Provirus GFP neo IRES SD SA Y Transgene m7G- -An RNA Genome GFP neo IRES SD SA Transgene Spliced Message m7G- -An GFP neo IRES MCS- multiple cloning sitem7G- 7-methyl guanosine cap SD- splice donor SA- splice acceptor LTR- long terminal repeats An- poly-A tail Y- packaging signal