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This study investigates the variation in abundance and diversity of microbial genes in agricultural soil across different stages from planting to harvesting in winter. Phylogenetic analyses of bacterial genes were conducted to understand the evolutionary relationships in the soil microbiome. The study focuses on the 16S rRNA genes of Variovorax asfA in bulk soil, with specific sampling points at Buinen and Valthermond soils during different plant growth stages (young, flowering, senescence). The study also includes before planting and after harvesting stages. The Neighbor-Joining method was used for phylogenetic analysis, with Kimura 2-parameter method for evolutionary distance calculation and bootstrap values for robustness.
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Supplemental Material Figure S1 – Abundance of 16S bacterial(A), Variovorax asfA (B) genes in the bulk soil per sampling in the B and V soils. Buinen [B], Valthermond [V], young plant [s], flowering [f], senescence [sn], bulk [b], before planting [p]], after harvesting in winter [w] Figure S2 – Phylogenetic analysis of ß-proteobacterial 16S rRNA genes retrieved from cultivar P (A) and A (B) in B soil at flowering stage. The evolutionary history was inferred using the Neighbor-Joining method. The evolutionary distances were computed using the Kimura 2- parameter method and bootstrap values (1000 repetitions). Figure S3 – Phylogenetic analysis of ß-proteobacterial 16S rRNA genes retrieved from Buinen bulk soil at flowering stage. The evolutionary history was inferred using the Neighbor-Joining method. The evolutionary distances were computed using the Kimura 2- parameter method and bootstrap values (1000 repetitions).