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SUPPLEMENTAL FIGURE S1

SUPPLEMENTAL FIGURE S1. A. -. ATP g S ( mM ). -. -. -. -. -. 2. 2. -. GTP(mM ). -. .5. .5. -. -. .5. -. -. GMP(mM ). -. -. -. .5. .5. -. .5. -. Cu 2+ (μM). 10. -. 10. -. 10. 10. 10. 1. NR. OH. T1. 2. 3. 4. 5. 6. 7. 8. B. 0.6. G45. G43. 0.5.

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SUPPLEMENTAL FIGURE S1

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  1. SUPPLEMENTAL FIGURE S1 A - ATPgS (mM) - - - - - 2 2 - GTP(mM) - .5 .5 - - .5 - - GMP(mM) - - - .5 .5 - .5 - Cu2+(μM) 10 - 10 - 10 10 10 1 NR OH T1 2 3 4 5 6 7 8 B 0.6 G45 G43 0.5 G42 0.4 G37 G36 Fraction cleaved 0.3 0.2 G29 0.1 0 GTP - 0.5mM 0.5mM GMP - - - ATPgS - - - 0.5mM - - - Cu2+ 10mM - 10mM 0.5mM - 0.5mM 0.5mM Supplemental Figure S1.Weak GMP interaction with ribozyme in presence of Cu2+ revealed by in-line probing. ILP reactions were performed for 10 hrs as in Figure 4 to monitor interactions with GMP. ATP or ATPS was added prior to GTP or GMP when more than one nucleotide was present. B. Integrated ILP cleavage signal at position A32 under the indicated conditions. 2mM - 2mM - 10mM - 10mM 10mM

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