Clostridium botulinum

2. Clostridium botulinum • Bacteria: Anaerobic, spore-forming, motile GPR • Source: Soils, sediments, intestinal tracts of fish/mammals, gills and viscera of crabs and other shellfish • Illness: Intoxication (heat-labile neurotoxin) • Symptoms: Weakness, vertigo, double vision, difficulty in speaking, swallowing and breathing, respiratory paralysis • Foods: Semi-preserved seafood, improperly canned foods • Transmission: Spores present in raw foods • Control: Proper canning, aw <0.93, pH <4.7

3. Clostridium perfringens • Bacteria: Anaerobic, spore-forming, nonmotile GPR • Source: Soil, dust, intestinal tract of animals and humans • Illness: Infection (toxin released on sporulation) • Symptoms: Intense abdominal cramps and diarrhea • Foods: Temperature abuse of prepared foods such as meats, meat products, and gravy • Transmission: Spores present in raw foods • Control: Proper time/temperature control; preventing cross-contamination of cooked foods

4. Bacillus cereus • Bacteria: Facultatively aerobic, spore-forming, motile GPR • Source: Soil, dust, raw foods • Illness: 1) diarrheal type (infection, heat-labile toxin); 2) emetic type (intoxication, heat-stable toxin) • Symptoms: 1) profuse watery diarrhea, abdominal pain; 2) vomiting, nausea • Foods: 1) vegetables, salads, meats, casseroles; 2) rice and pasta • Transmission: Spores present in raw foods • Control: time/temperature; reheat cooked foods to >165o F

5. Brucella abortis, B. suis • Bacteria: Aerobic, nonmotile, GNR • Source: Domestic and wild animals • Illness: Infection (undulant fever) • Symptoms: Sweats, chills, weakness, aches, joint pains • Foods: Raw milk, infected meat • Transmission: Infected animals • Control:Pasteurize milk, cook meats

6. Campylobacter jejuni • Bacteria: Microaerophilic, motile GNR • Source: Intestines of poultry, livestock, domestic animals; streams and ponds • Illness: Infection (gastroenteritis) • Symptoms: Diarrhea, abdominal pain, headache, weakness, fever • Foods: undercooked chicken & hamburger, raw milk & clams • Transmission: Contaminated foods & water; cross-contamination; person to person • Control: Proper cooking, proper hand and equipment washing, sanitary food handling practices

7. Pathogenic Escherichia coli O157:H7 • Bacteria: Facultative anaerobic, motile or nonmotile GNR • Source: Intestines of animals and poultry • Illness: Hemorrhagic colitis (HC), hemolytic uremic syndrome (HUS), thrombotic thrombocytopenic purpura (TTP) • Symptoms: HC) diarrhea & vomiting, HUS) diarrhea & acute renal failure, TTP) diarrhea, GI hemorrhage, blood clots in brain • Foods: Meat, poultry, potatoes, raw milk • Transmission: Cross-contamination, sewage pollution of coastal waters • Control: Proper cooking, temperature control, preventing cross-contamination, proper personal hygiene

8. Listeria monocytogenes • Bacteria: Microaerophilic, motile, GPR • Source: Widespread in the environment • Illness: Infection • Symptoms: Mild flu-like symptoms to meningitis, abortions, septicemia, and death • Foods: Coleslaw, raw milk, Mexican style soft cheese, smoked mussels • Transmission: Cross-contamination from raw to cooked food, contaminated raw foods • Control: Proper cooking, preventing cross-contamination, pasteurizing milk

9. Salmonella spp. • Bacteria: Facultative anaerobic, motile, GNR • Source: Intestine of mammals, birds, amphibians and reptiles • Illness: Infection (gastroenteritis) • Symptoms: Nausea, vomiting, abdominal cramps, fever • Foods: Poultry, poultry salads, meats, dairy products, egg products • Transmission: Cross-contamination, human contamination, sewage pollution of coastal waters • Control: Proper cooking, temperature control, preventing cross-contamination, personal hygiene

10. Shigella spp. • Bacteria: Facultative anaerobic, nonmotile, GNR • Source: Intestine of humans and primates • Illness: Infection (gastroenteritis) • Symptoms: Mild diarrhea, fever, abdominal cramps, severs fluid loss • Foods: Water, milk, salads, lettuce, watermelon, beans, spaghetti • Transmission: Contamination from workers, sewage pollution of coastal waters, contamination of seafood after harvest • Control: Personal hygiene, preventing human waste contamination of water supplies, preventing ill people or carriers from working with food

11. Pathogenic Staphylococcus aureus • Bacteria: Facultative aerobic, nonmotile, GPC • Source: Humans and animals, air, dust, sewage • Illness: Intoxication (gastroenteritis) • Symptoms: Nausea, vomiting, abdominal cramps, watery or bloody diarrhea, fever • Foods: Meats, poultry, eggs, dairy products, seafood • Transmission: Contamination of food by workers or equipment • Control: Time/temperature control, personal hygiene, sanitation

12. Vibrio cholerae • Bacteria: Facultative aerobic, motile, curved GNR • Source: Naturally occurring in estuaries, bays and brackish water • Illness: Infection (cholera or gastroenteritis) • Symptoms: 01: watery diarrhea, vomiting, abdominal cramps; non-01: Diarrhea, abdominal cramps, fever • Foods: Molluscan shellfish • Transmission: Contaminated water, cross-contamination from raw to cooked seafood, consumption of contaminated raw seafood • Control: Proper cooking, preventing cross-contamination, Harvesting from approved waters

13. Vibrio parahaemolyticus • Bacteria: Facultative aerobic, motile, curved GNR • Source: Naturally occurring in estuaries and other coastal areas throughout the world • Illness: Infection (gastroenteritis) • Symptoms: Diarrhea, abdominal cramps, nausea, vomiting, headache • Foods: Raw, improperly cooked, or cooked and contaminated fish and shellfish • Transmission: Cross-contamination from raw to cooked seafood, consumption of raw seafood • Control: Proper cooking, preventing cross-contamination of cooked seafood

14. Vibrio vulnificus • Bacteria: Facultative aerobic, motile, curved GNR • Source: Naturally occurring marine bacterium • Illness: Infection (wounds, gastroenteritis, septicemia) • Symptoms: Skin lesions, septic shock, fever, chills, nausea • Foods: Raw oysters, clams and crabs • Transmission: Cross-contamination from raw to cooked seafood, consumption of raw seafood • Control: Proper cooking, preventing cross-contamination of cooked seafood

15. Yersinia enterocolitica • Bacteria: Facultative aerobic, motile, GNR • Source: Soil, water, domesticated and wild animals • Illness: Infection (gastroenteritis) • Symptoms: Diarrhea, vomiting, abdominal pain, fever • Foods: Meats, oysters, fish, raw milk • Transmission: Cross-contamination from raw to cooked food, poor sanitation, time/temperature abuse • Control: Preventing cross-contamination, proper sanitation and food handling practices

16. Viruses • Hepatitis A and E • Norwalk virus group

17. Hepatitis A • Source: Human intestine • Illness: Infection • Symptoms: Fever, malaise, nausea, abdominal discomfort, jaundice • Foods: Cold cuts, sandwiches, fruits, fruit juices, milk and milk products, vegetables, salads, shellfish, iced drinks • Transmission: Fecal contamination of food or water • Control: Proper cooking, preventing cross-contamination, good sanitation, employee hygiene

18. Norwalk Virus Group • Source: Human intestines • Illness: Infection • Symptoms: Self-limiting and mild; nausea, vomiting, diarrhea, abdominal cramps, fever • Foods: Salad ingredients, raw or insufficiently cooked clams and oysters • Transmission: Fecal contamination of food or water • Control: Proper cooking, good sanitation, employee hygiene, preventing cross-contamination

19. Anisakis simplex • Parasite: Nematode (herring worm) • Source: Raw or undercooked fish (salmon, tuna, herring, mackerel, squid, anchovies) • Illness: Infection • Symptoms: Tickling sensation in throat to acute abdominal pain and nausea • Transmission: Consumption of raw or undercooked fish • Control: Proper cooking of fish, commercial freezing of fish to be consumed raw

23. Ascaris lumbricoides • Parasite: Nematode • Source: Human intestines, insufficiently treated sewage-fertilizer • Illness: Infection • Symptoms: Vague digestive tract discomfort, migration of nematode into throat/mouth/nose • Transmission: Infected food handlers, sewage fertilized fruits and vegetables • Control: Good sanitation, employee hygiene, proper treatment of sewage used for fertilizer

24. Pseudoterranova dicepiens • Parasite: Nematode (codworm) • Source: Raw or undercooked fish (cod, pollock, haddock) • Illness: Infection • Symptoms: Tickling sensation in throat to acute abdominal pain and nausea • Transmission: Consumption of raw or undercooked fish • Control: Proper cooking of fish, commercial freezing of fish to be consumed raw

25. Trichinella spiralis • Parasite: Nematode • Source: Pork and bear meat • Illness: Infection (Trichinosis) • Symptoms: Fever, muscle soreness, pain and swelling around the eyes. Chest pain may be experienced since the parasite may become imbedded in the diaphragm. • Transmission: Raw or improperly cooked infected pork or bear meat • Control: Thoroughly cook pork and other potentially infected meats, cook garbage fed to hogs, avoid cross-contamination of beef with pork

26. Diphyllobothrium latum • Parasite: Tapeworm • Source: Raw freshwater or anadromous fish • Illness: Infection • Symptoms: Abdominal distention, flatulence, intermittent abdominal cramping and diarrhea. • Transmission: Consumption of raw or undercooked fish • Control: Proper cooking of fish

29. Taenia solium, T. saginata • Parasite: Tapeworm (T. solium, pork tapeworm; T. Saginata, beef tapeworm) • Source: Swine or beef • Illness: Infection; worms attach to lining of small intestine and can grow large enough to block the intestinal tract • Symptoms: Nausea, epigastric fullness, and vomiting. Central nervous system disorders may arise in intermediate hosts of T. solium • Transmission: Raw or improperly cooked infected pork or beef • Control: Proper cooking of pork and beef

30. Cryptosporidium parvum • Parasite: Protozoa • Source: Cows, goats, sheep, deer, elk • Illness: Infection (intestinal, tracheal, or pulmonary cryptosporidiosis) • Symptoms: Intestinal - severe watery diarrhea or asymptomatic; pulmonary and tracheal - coughing and low-grade fever • Transmission: Contaminated food handler, person-to-person, contaminated water supplies, salad vegetables fertilized with manure • Control: Personal hygiene, prohibit fertilizing salad vegetables with manure, boil or filter contaminated water

31. Entamoeba histolytica • Parasite: Protozoa • Source: Intestinal tract of humans and primates • Illness: Infection • Symptoms: 1) none, 2) vague gastrointestinal distress, or 3) dysentery • Transmission: Fecal contamination of drinking water and foods, person-to-person, infected food handlers • Control: Good sanitation, employee hygiene

32. Giardia lamblia • Parasite: Protozoa • Source: Domestic and wild animals • Illness: Infection • Symptoms: Diarrhea (the most common cause of non-bacterial diarrhea in North America) • Transmission: Contaminated water, infected food handlers, contaminated vegetables eaten raw • Control: Water treatment, good sanitation, employee hygiene, good food handling practices

35. Types of Naturally Occurring Chemical Hazards • Mycotoxins (e.g., aflatoxin) • Scombrotoxin • Ciguatoxin • Shellfish toxins • Paralytic shellfish poisoning (PSP) • Diarrhetic shellfish poisoning (DSP) • Neurotoxic shellfish poisoning (NSP) • Amnesic shellfish poisoning (ASP)/Domoic Acid • Other marine toxins • Gempylotoxin • Tetrodotoxin

36. Scombrotoxin (Histamine) • Toxin: Histamine • Source: Improperly handled (time/temperature abuse) mahi mahi, tuna, bluefish, sardines, amberjack, mackerel • Range: Worldwide • Symptoms: Metallic or peppery taste, nausea, vomiting, abdominal cramps, diarrhea, swelling and flushing of face, headache, dizziness, heart palpitations, hives, rapid and weak pulse, thirst, difficulty swallowing • Control: Proper chilling and temperature control after capture • FDA Guideline: 50 ppm histamine in raw, frozen tuna and mahi mahi; canned tuna; and related species

37. Ciguatera Fish Poisoning • Toxin: Ciguatoxins (4 toxins) • Source: Certain species of tropical and subtropical fish feeding on algae (Gambierdiscus spp.) • Range: Tropical and subtropical waters worldwide • Symptoms: Diarrhea, abdominal pain, nausea, vomiting, abnormal or impaired skin sensations, vertigo, lack of muscle coordination, cold/hot sensation reversal, muscular pain and itching • Control: No tests available; obtain fish from safe harvest areas • FDA Guideline: None established

38. Paralytic Shellfish Poisoning • Toxin: Saxitoxins (18 toxins) • Source: Contaminated molluscan shellfish feeding on algae (Alexandrium, Pyrodinium, Gymnodinium spp.) • Range: Tropical to temperate waters worldwide • Symptoms: Numbness and burning or tingling sensation of lips and tongue spreading to face and fingertips, general lack of muscle coordination in arms, legs, neck • Control: Obtain molluscan shellfish from waters that have been approved for harvest • FDA Guideline: 0.8 ppm saxitoxin equivalent (80ug/100g) in all fish

39. Diarrhetic Shellfish Poisoning • Toxin: Okadaic acid and its derivatives • Source: Molluscan shellfish feeding on algae (Dinophysis and Prorocentrum spp.) • Range: Japan, southeast Asia, Scandinavia, western Europe, Chile, New Zealand, eastern Canada • Symptoms: Diarrhea, nausea, vomiting, abdominal pain, cramps • Control: Obtain molluscan shellfish from waters that have been approved for harvest • FDA Guideline: 0.2 ppm okadaic acid plus 35-methyl okadaic acid (DXT 1) in all fish

40. Neurotoxic Shellfish Poisoning • Toxin: Brevetoxins (3 toxins) • Source: Molluscan shellfish feeding on algae (Gymnodinium breve) • Range: Gulf of Mexico and southern Atlantic coast in U.S. New Zealand • Symptoms: Tingling of the face and spreading to other parts of the body, cold/hot sensation reversal dilation of pupils, feeling of inebriation • Control: Obtain molluscan shellfish from waters that have been approved for harvest • FDA Guideline: 0.8 ppm brevetoxin-2 equivalent (20 mouse units/100g) in clams, mussels and oysters

41. Amnesic Shellfish Poisoning • Toxin: Domoic acid • Source: Molluscan shellfish (mussels) feeding on algae (Pseudonitzschia spp.), viscera of Dungeness crab and anchovies • Range: Northeast and northwest coasts of North America • Symptoms: Intestinal distress, facial grimace or chewing motion, short-term memory loss, difficulty breathing • Control: Obtain molluscan shellfish from waters that have been approved for harvest • FDA Guideline: 20 ppm domoic acid in all fish; 30 ppm domoic acid in viscera of Dungeness crab

42. Gempylotoxin • Toxin: Oil contained in the flesh and bones of specific species • Source: Gemplids, escolars or pelagic mackerels (escolar; oilfish, castor oil fish or purgative fish; snek) • Range: Almost worldwide • Symptoms: Diarrhea, generally without pain or cramping • Control: Avoid specific fish species • FDA Guideline: Escolar should not be imported

43. Tetrodotoxin • Toxin: Tetrodotoxin • Source: About 80 species of puffer fish, blowfish or fugu • Range: Pacific, Atlantic and Indian Oceans • Symptoms: Numbness and tingling of the mouth, weakness, paralysis, decreased blood pressure, quickened and weakened pulse. Death can occur within 30 minutes. • Control: Do not eat puffer fish or avoid improperly prepared pufferfish • FDA Guideline: Puffer fish may not be imported except under strict certification requirements and specific authorization from FDA

44. Tetramine • Toxin: Tetramine • Source: Salivary gland of whelk (Neptunia spp.) • Range: Primarily the Sea of Japan • Symptoms: Intense headache, dizziness, nausea and vomiting • Control: Remove salivary glands before consumption • FDA Guideline: Remove the salivary glands of Neptunia spp.