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中文: 發育生物學 英文: Developmental Biology 授課教師: 劉薏雯

中文: 發育生物學 英文: Developmental Biology 授課教師: 劉薏雯. This course aims to help the students understand how life develops from a single fertilized egg to an organism with complicated and integrated functions. By the end of the course, the students will be familiar with:

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中文: 發育生物學 英文: Developmental Biology 授課教師: 劉薏雯

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  1. 中文: 發育生物學 英文: Developmental Biology 授課教師: 劉薏雯

  2. This course aims to help the students understand how life develops from a single fertilized egg to an organism with complicated and integrated functions.

  3. By the end of the course, the students will be familiar with: (1)The development of model organisms including Drosophila, zebrafish, mouse etc. (2)The important molecular and cellular mechanisms that govern the developmental processes. (3)How congenital diseases will occur while normal development is perturbed.

  4. The frontiers of developmental biology, such as “cloning” and “stem cell”.

  5. 4.8 Cloned mammals, whose nuclei came from adult somatic cells

  6. 4.9 The kitten “CC” (A) is a clone produced using somatic nuclear transfer from “Rainbow” (B)

  7. Scott F. Gilbert Developmental Biology. 8th edition www.devbio.com 上課時間

  8. 隨堂考試 (40%) 書面報告 (40%) 口頭報告 (10%) 上課出席 (10%)

  9. The foundations of developmental biology: Observations of living organisms in nature! (not from test tubes…) The ability to observe and record the anatomical details of an organisms Artist as well as natural scientist…

  10. Developmental biology in the post-genomics era: The relationship between “Gene” and “Development” Gene expression Molecular biology and biochemistry Knock-out and knock-in techniques Genetics Genomics

  11. Studying the functions of a gene by Knock-out technologies Homologous recombination Double-stranded RNA (dsRNA) Antisense morpholino oligo

  12. 4.19 Technique for gene targeting

  13. 4.21 Use of antisense RNA to examine the roles of genes in development

  14. 4.22 The dsRNA-mediated gene silencing procedure

  15. 4.23 Injection of dsRNA for E-cadherin into the mouse zygote blocks E-cadherin expression

  16. Antisense Morpholino Oligonucleotide a targeted knockout in zebrafish

  17. Studying the functions of a gene by Knock-in technologies Reporter transgenic lines (For monitoring the expression of a gene or tracing a specific lineage. ) Chimeric mice Transgenic animal for pharmaceutical industry

  18. 4.17 Insertion of new DNA into embryonic cells

  19. 4.18 Production of transgenic mice

  20. 4.18 Production of transgenic mice

  21. Other approaches for understanding the function of a gene: Spatio-temporal expression RNA localization techniques for understaning differential gene expression

  22. 4.12 The reverse transcriptase-polymerase chain reaction technique for determining whether a particular type of mRNA is present

  23. 4.13 Microarray technique

  24. 4.15 In situ hybridization showing the expression of the Pax6 gene in the developing mouse eye

  25. 4.16 Whole-mount in situ hybridization localizing Pax6 mRNA in early chick embryos

  26. 4.16 Whole-mount in situ hybridization localizing Pax6 mRNA in early chick embryos

  27. Model organisms of genetics and genomics: Mouse Drosophila Zebrafish C. elegans Arabidopsis Other model organisms for developmental biology: Chick Amphibians

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