Aquaculture Disease Processes

1. Aquaculture Disease Processes Dr. Craig Kasper FAS 2253/FAS 2253L

2. Description of Syllabus • Course Number and Title: FAS 2253/FAS 2253L, Aquacultural Disease Processes • Lecture Time/Location: TTH/9:30-1045am/BSC 212 • Lab Time/Location: TH/11:00a-12:40p/BSC 212 • Instructor: Dr. Craig Kasper, BHUM 111, 253-7881, ckasper@hccfl.edu

3. Description of Syllabus • Exposure to fundamental and current disease/health issues pertaining to the production of aquaculture crops • Prevention of diseases via practical diagnosis and real-world decision making!! • Covers: anatomy and physiology, immunology, virology, bacterial diseases, nutritional diseases, parasitology, mycoses, larval diseases and general health management

4. Syllabus • Text: Fish Disease and Diagnosis (Noga, Blackwell Publishers). • Additional readings will be on reserve in the library. • Course will consist of weekly two-hour lectures (2) followed by a two-hour practical lab. • You will need “grubby” clothes on lab day • No open-toed shoes in lab!! • Labs may require observation and checking on samples outside class period

5. Syllabus: lecture outline Date Topic 8/24 Introduction to Disease 8/29 Signs vs. Symptoms? What’s the difference? 8/31 Immune Response in Aquaculture Animals, Part 1 9/5 Immune Response in Aquaculture Animals, Part 2 9/7 Diseases of a Non-infectious Nature (Nutritional) 9/12 Exam 1 9/14 Common Viral Pathogens of Aquaculture Organisms, Part 1 9/19 Common Viral Pathogens of Aquaculture Organisms, Part 2 9/21 Common Bacterial Pathogens of Aquaculture Organisms, Part 1 9/26 Common Bacterial Pathogens of Aquaculture Organisms, Part 2 9/28 Exam 2 10/3 Probiotic Bacteria (Part 1) 10/5 Probiotic Bacteria (Part 2) 10/10 Molds and Fungi (Part 1) 10/12 Molds and Fungi (Part 2) 10/17 Exam 3

6. Date Topic 10/19 Protozoans and Parasites Part 1 10/24 Protozoans and Parasites Part 2 10/26Exam 4 10/31 Aquaculture Health Programs 11/2 Design of High Health Facilities/HACCP/Biosecurity 11/7 Practical Considerations 11/9 Regulations, Drugs and the FDA 11/14Exam 5 11/16 Treatments 11/21 Ethics in treating fish. 11/23 Case Study (Real World Example) 11/28 Case Study (Real World Example) 11/30 Thanksgiving Break (No Class) 12/2 Presentations 12/7 Presentations 12/9 Final Exam (non-cumulative)

7. Lecture 1: Introduction to Disease • What is disease? • Types of diseases • Dynamics of infectious disease • Epizootiology of infectious diseases • What you have to do to be a disease agent • Disease reservoirs • Transmission • The host • Stages in an epizootic

8. What is Disease? • Definition: any alteration of the body or one of its organs so as to disturb normal physiological function • opposite of health = unhealthy or dysfunctional

9. Why are diseases of such concern in aquaculture? • 1990: WSSV, a virus, devastates shrimp culture in China, $600 million lost • 1971: Flexibacter columnaris, a bacterium, kills 14 million wild fish in Klamath Lake • the Idaho trout industry loses 10 cents on every dollar made to disease (death, weight loss) • future of finfish and shrimp culture may hinge on our ability to control vibriosis* *more on “vibrio” in a later lecture!

10. Types of Diseases • infectious: diseases due to the action of microorganisms (animal or plant): • viruses: CCV, WSSV, TSV, YHV • bacteria: Vibrio sp. • protozoans • metazoans • fungi: Saprolegnia sp. • crustaceans: O. Isopoda

11. Types of Diseases 2) non-infectious: diseases due to non-living causes (environmental, other) • even a moderately adverse environment can lead to stress, stress leads to epizootics • a very adverse environment can cause disease and mortalities directly (e.g., nitrogen gas bubble disease, brown blood disease) • the “other” category refers to nutritional, genetic and developmental diseases

12. Types of Diseases 3) treatable vs. non-treatable • non-treatable diseases are some of the worst • include pathogens such as viruses, drug-resistant bacteria, myxozoans • white spot syndrome virus (shrimp) has no known treatment • Vibrio sp.: because of rampant over-use of antibiotics in Central America, South America, new, more virulent strains are developing

13. Dynamics of Infectious Diseases • First mode of infection demonstrated by Robert Koch (1876) and his work with Bacillus anthracis(anthrax) • reached epidemic proportions in cattle, sheep and other domesticated animals • also can occur in man (as we are well aware!) • Koch showed that a bacterium caused the disease by using the following method:

14. Koch’s Method (Postulates) • 1) find the organism common to all infected animals, demonstrate its absence in healthy ones • 2) isolate the organism in pure culture • 3) reproduce the disease in suitable experimental animals • 4) reisolate the same organism from experimentally infected animals

15. Dynamics of Disease: Germ Theory • Koch’s work lead to what is known as the germ theory: germs cause disease • if you have germs you are diseased • Renes Dubos (1955) refined the concept in the following statement: “There are many situations in which the microbe is a constant and ubiquitous component of the environment but causes disease only when some weakening of the patient by another factor allows infection to proceed unrestrained, at least for a while. Theories of disease must account for the surprising fact that, in any community, a large percentage of healthy and normal individuals continually harbor potentially pathogenic microbes without suffering any symptoms or lesions.”

16. Dynamics of Disease: stress • Definition: any stimulus (physical, chemical or environmental) which tends to disrupt homeostasis in an animal. • The animal must then expend more energy to maintain homeostasis: less energy to combat disease • Aquatic organisms are fundamentally different from terrestrials: they are immersed in their environment, can’t go somewhere else • some disease agents are almost always present in the water (ubiquitous) • examples: Aeromonas sp., Pseudomonas sp., Vibrio sp.

17. Dynamics of Infectious Disease: how it occurs • Three-set model: • susceptible host • pathogenic agent • environment unfavorable to host/favorable to agent • exceptions??: extremely large numbers of bacteria, extremely virulent agent • stress throws a wrench into it all

18. Dynamics of Infectious Diseases • infection  parasitism  disease (infection can result from parasitism, but neither necessarily results in disease • symbiosis: any association between 2 species involving an exchange of matter and energy • commensalism: symbiosis in which one partner benefits, the other is neutral • parasitism: symbiosis in which the parasite (usually smaller) is metabolically dependent on the host (larger); some harm intuitive, but not necessary

19. Epizootiology of Infectious Diseases: terminology • epidemiology: branch of medicine describing occurrence, distribution and types of diseases in populations of animals at distinct periods of time and at particular places (usually refers to humans) • epizootiology: same as above (non-human) • epidemiology is the study of the who, what, when, where, how and why of disease outbreaks

20. Epizootiology of Disease: outbreak terminology • enzootic vs. epizootic (endemic vs. epidemic) • incidence: frequency of disease in a population over time in relation to the population in which it occurs (cases/yr) • rate: number of new cases per number of population (per thousand) • prevalence: the expression of the frequency of a disease at a particular point in time in relation to the population in which it occurs (%) • proportion: number affected/population • mortality: the percentage expression of the frequency of deaths over a period of time in the total population (not a rate, a proportion)

21. How to Become a Disease Agent:6 Commandments of Parasitism • Find a proper host • Somehow get in or access inside • Find a home • Be fruitful and multiply • Get out once done or developed • Be transmitted to a new host • all this obviously involves specificity in the host:parasite relationship

22. Host:Parasite Specificity • Specificity is required for steps 1 and 3, above (find a proper host, find a home inside) • host specificity example: Shasta rainbow trout are highly susceptible to Ceratomyxa shastawhile Crystal Lake individuals are completely resistant • reason: physiological specificity (the host must meet all of the metabolic requirements of the agent without destroying it immunologically)

23. Host:Parasite Specificity • Another example: Why are centrarchids infected with black spot metacercariae while walleyes aren’t? • Answer: ecological specificity -- the host and agent must overlap in time and space • Another type of specificity: tissue specificity

24. For Next Time…. • Will continue with introduction to disease • Check books on reserve in the library…. • Lab tonight: fish interna/exeternal anatomy, we provide dissection kits, etc.

25. Potential for Disease via Infection: contributors • numberof organisms (overwhelming) • infectivity (ability to get in) • virulence (ability to produce disease) • susceptibility of the host • agent’s ability to overcome host’s defenses • level of stress (REM!) • probablility of disease (Theobald Smith Model) = (# agents x virulence of agents)÷(resistance of host)

26. Possible Fates of an Agent within its Host • host dies: agent proliferates, overwhelms host, good parasites don’t do this, $$$$$ • host lives: largely dependent on stress • host gets sick, but recovers (defense worked) • host doesn’t get sick (agent not virulent, wrong host) • survivors: • agent either eliminated or • carrier state established (host infected, but no obvious disease, big problem) • latent(not easily observed) • patent (ongoing/observable)

27. Mortality Curves: bell shaped • Infectious agent or toxic substance moves into the population and then, after time, no longer affects events in population. • Transmission is horizontal with width of curve proportional to incubation time and period of communicability. Agent??: typically bacterial

28. Mortality Curves: sigmoidal • Slight deviation from bell-shaped curve due to lag period in course of disease (lag phase of growth) • Also, periods in which the disease is not communicable. lag Agent??: typically bacterial

29. Mortality Curves: point source • Population at risk was exposed to agent at a single point in time. • All susceptible members affected. • Highly virulent infectious type disease of toxic agent • Exposure to toxin. Agent??: chemical, viral

30. Mortality Curves: plateau- shaped • Indicates exposure over a long period of time • slow incubation • slow transmission Agent??: possibly nutritional

31. Mortality Curves: multiple spiked • Due to frequent but intermittent exposure to disease agent • Data usually or eventually indicate plateau effect • Must take care re frequency of sample Agent??: physical parameter (e.g., low D.O.)

32. Theoretical Cumulative Mortality Patterns

33. Degree of Infection • Acute: high degree of mortality in short period of time, external signs might be completely lacking (e.g., CCV, IHNV, TSV, WSSV) • Chronic: gradual mortality, difficult to detect a peak (Aeromonas septicemia, furunculosis) • Latent: disease agent present, but host shows no outward sign, little or no mortality, sometimes associated with secondary pathogen/infection (CCV and Edwardsiella ictaluri)

34. The Reservoir Concept • reservoir: the sum of all sources of the agent, the natural habitat of the agent, where the agent comes from • The size of the reservoir is proportional to the chance of spread of a pathogen • transient reservoir: situation in which the epizootic displays a seasonal pattern of either cases or carriers • permanent reservoir: usually associated with disease in which chronic carriers are shown • good example: water supply, itself

35. Transmission • Definition: mode of transfer of disease to a new host • Method 1)direct transmission: from one host to another, either a) vertically or b) horizontally • vertical transmission: from parent to offspring • via male (Girodactylus, trematode in pipefish) • via female (IHN) • horizontal transmission: from one member of a population to another, one offspring to another • contact: typically water borne (e.g., fish to fish) • ingestion of agent or of infected aquatic

36. Transmission • Method 2)indirect transmission: infection via an inanimate vehicle, vector or intermediate host • vehicle: an inanimate object such as handling equipment (nets, waders, etc.) or feed (e.g., aflatoxin) • vector or intermediate host: animate object • mechanical: vector is not essential to life cycle of agent • biological: agent spends some part of life cycle in vector(e.g., water boatman and WSSV)

37. Disease Transmission: getting in the door Portals of entry, not as easy as they sound: • ingestion: e.g., Ceratomyxa shasta, BKD, Myxobolus cerebralis • gill lamellae: e.g., Schizamoeba salmonis, Ichthyobodo necatur • lesions: bacteria (Vibrio sp.), fungi (Saprolegnia sp.) • active penetration: some metazoans, dinoflagellates

38. The Host • The ability of a host to acquire a disease agent and demonstrate disease symptoms can be expressed both qualitatively and quantitatively • qualitatively: resistance(ability of a host to withstand the effects of an agent; e.g., Litopenaeus stylirostris to TSV) • quantitatively: susceptibility (a measure of the host’s ability to tolerate an agent)

39. Resistance: Primary Factors Physical barriers, inflammation, natural immunity, acquired immunity • physical barriers: refers to innate characteristic of animal body to penetration (e.g., mucous slime layer, intact skin, mucous membranes, exoskeleton) • for fish, the mucous slime layer itself displays an immune response (phagocytic properties, antibodies)

40. Resistance: Primary Factors • inflammation: basic response to any wound, designed to seal off the area and reduce further infection/damage • manifestations (humans) include swelling, reddening, loss of function, heat, pain • manifestations (fish) possibly include heat and pain • histological changes: local edema(swelling); infiltration of neutrophils (type of white blood cell produced in bone marrow) , lymphocytes (lymph proteins), macrophages; fibroplasia (formation of fibrous tissue in wounds)

41. Resistance: Primary Factors 3) Immune Response • natural immunity: inherited (discussed in detail later) • acquired immunity: either active or passive • active: obtains antibody via contact with antigen • passive: antibody obtained via donor (vaccination) • discussed in following lecture

42. Resistance: secondary factors • Secondary factors associated with disease resistance are either environmental in nature orsomatic (associated with host, itself) • environmental factors: mainly stress resulting from deviation in temperature, dissolved oxygen, ammonia; inadequate nutrition; mechanical, etc. • somatic factors: age, sex, species (e.g., IPN affects only largest fry, potential for exposure, immune experience via exposure, black spermataphore, TSV)

43. Stages in Epizootic • REM: epizootic is an outbreak of disease • incubatory: agent has penetrated host barrier, found home and multiplying • clinical or subclinical: host adversely affected (manifestations) • depression (reduced activity) • color change • interrupted feeding behavior • body contortions • respiratory change • mortality

44. Stages in Epizootic • terminal: host either dies or recovers • exception: in some very acute, highly pathogenic diseases (e.g., MBV) death may occur so fast that obvious signs don’t develop • NEXT: Immune Response in Aquaculture Organisms