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from Shendure and Hanlee (2008) Nat. Biotech. 26:1135

from Shendure and Hanlee (2008) Nat. Biotech. 26:1135. 454 platform – pyrosequencing. from Shendure and Hanlee (2008) Nat. Biotech. 26:1135. Pyrosequencing: several views. APS = adenosine phosphosulfate.

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from Shendure and Hanlee (2008) Nat. Biotech. 26:1135

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  1. from Shendure and Hanlee (2008) Nat. Biotech. 26:1135

  2. 454 platform – pyrosequencing from Shendure and Hanlee (2008) Nat. Biotech. 26:1135

  3. Pyrosequencing: several views APS = adenosine phosphosulfate Apyrase, a nucleotide degrading enzyme, continuously degrades unincorporated dNTPs and excess ATP. When degradation is complete, another dNTP is added. from www.columbia.edu/cu/biology/courses/w3034/.../lec20_06.ppt

  4. Solexa sequencing from Shendure and Hanlee (2008) Nat. Biotech. 26:1135

  5. Fig. 12-15, Garrett and Grisham, Biochemistry, 3rd ed.

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