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Genome Sequence Analysis of Chlamydia trachomatis Mopn_tet_14

Genome Sequence Analysis of Chlamydia trachomatis Mopn_tet_14. Anjali Menon Rockey Lab Dryden Hall, OSU. Chlamydia. Objectives. Part 1 Grow and amplify mopn_tet_14 Send to the Illumina machine for sequencing . Objectives. Part 2

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Genome Sequence Analysis of Chlamydia trachomatis Mopn_tet_14

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  1. Genome Sequence Analysis of Chlamydia trachomatis Mopn_tet_14 Anjali Menon Rockey Lab Dryden Hall, OSU

  2. Chlamydia

  3. Objectives Part 1 • Grow and amplify mopn_tet_14 • Send to the Illumina machine for sequencing

  4. Objectives Part 2 • Align gene sequences using MUSCLE, a multiple sequence alignment algorithm • Use Perl programming language to create a comparative database for known chlamydial genomes

  5. Methods and Materials • Glycerol stock of mopn_tet_14 • PCR • DNA Gels • Illumina Sequencing • Perl and VIM programming languages to create database for comparative analysis

  6. Process

  7. Process

  8. Process

  9. Future Projects

  10. Summary • Grew and amplified mopn_tet_14 • Sequenced, identified regions of uncertainty • Designed primers to cut individual regions • Confirmed mopn_tet_14 sequence • Used MUSCLE to align gene sequences • Started using Perl and VIM to organize sequences, and ultimately, to create a comparative database for completed chlamydial genomes

  11. Acknowledgements • HHMI • Kevin Ahern • OSU’s CGRB • Dr. Dan Rockey • Brendan Jeffrey • Kelsi Sandoz

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