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Phenotyping: Response to Water Deficits. Motivation Importance of root growth to seedling establishment Water is widespread limitation to productivity Water limitations common in seedling stage Little gene discovery work with roots
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Phenotyping: Response to Water Deficits • Motivation • Importance of root growth to seedling establishment • Water is widespread limitation to productivity • Water limitations common in seedling stage • Little gene discovery work with roots • Molecular markers for root traits have great potential for application • WUE identified as selection trait for other species • Growing importance of clonal forestry for industry
FBRC CCLONES Experiment • 60 clones from each of 15 elite, full-sib families • 8 ramets/clone planted at each of six field locations by FBRC cooperators in December 2002 • Two cultural treatments: "optimal" and low-intensity
Phenotyping Measurements - FBRC CCLONES population • Destructive root biomass and architecture measurements on 4 ramets/clone prior to planting (Dec 2002) • Measurements 1 year post-planting, all sites (21,600 plants) • shoot height • survival • disease incidence
Phenotyping Measurements - FBRC CCLONES population • Additional physiological measurements 1 year post-planting (2003) • Intensive blocks, two sites with contrasting soils, subset of 600 clones (4800 plants) • specific leaf area • late-summer foliar 12C:13C • pre-dawn water potential and RWC during water deficit periods in May and October