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The Effects of Pollution on Bioluminescent Bacteria

The Effects of Pollution on Bioluminescent Bacteria. By: Tobi Simon. Need. Long-term exposure Plants and Animals. http://www.peternjenga.com/blogs/greenkenya/wp-content/uploads/2009/03/natural-and-manufactured-air-pollution.png. Knowledge Base.

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The Effects of Pollution on Bioluminescent Bacteria

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  1. The Effects of Pollution on Bioluminescent Bacteria By: Tobi Simon

  2. Need Long-term exposure Plants and Animals http://www.peternjenga.com/blogs/greenkenya/wp-content/uploads/2009/03/natural-and-manufactured-air-pollution.png

  3. Knowledge Base http://nptel.iitm.ac.in/courses/Webcourse-contents/IIT-Delhi/Environmental%20Air%20Pollution/air%20pollution%20(Civil)/Module-1/images1/35.gif

  4. Knowledge Base Bioluminescent Bacteria http://www.mcbs.unh.edu/faculty/Whistler/images/image3.jpg

  5. Literature Review Bolster, Carl H. "Comparison of E. coli and C. jejuni Transport in Saturated Porous Media." Journal of Environmental Quality (2006).

  6. Literature Review • Alvarez, David A. "Chemical and Toxicologic Assessment of Organic Contaminants in Surface Water Using Passive Samples." Journal of Environmental Quality (2008).

  7. Literature Review • Vanhaecke, Erwin. "Bioluminescence Assay for Measuring the Number of Bacteria Adhering to the Hydrocarbon Phase in the BATH Test." Applied and Environmental Microbiology (1988).

  8. Literature Review • Frischer, M. E. "Bioluminescent Bacteria as Indicators of Chemical Contamination of Coastal Waters." Journal of Environmental Quality (2005).

  9. Purpose The effects of pollution on the amount of light emitted from bioluminescent bacteria.

  10. Hypothesis Alternate: Water with higher amounts of hydrocarbons will cause a decrease in the amount of light emitted from the bacteria Null: Water with hydrocarbons in it will have no effect on the amount of light emitted from the bacteria.

  11. Methodology Water is collected from different locations and a sample of each is tested for the amount of hydrocarbons present. Bioluminescent bacteria are placed into different samples of water Control No hydrocarbon in water Variable 1 Lowest amount of hydrocarbon in water Variable 2 Medium amount of hydrocarbon in water Variable 3 Highest amount of hydrocarbon in water After 24 hours the BLR will be calculated for each sample of water. Statistical Analysis: ANOVA followed by a Sheffe post hoc test

  12. Do Ability Organisms Procedures http://www.abdn.ac.uk/staffpages/uploads/bch052/aglover-bac.jpg

  13. Budget

  14. Bibliography • Bolster, Carl H. "Comparison of E. coli and C. jejuni Transport in Saturated Porous Media." Journal of Environmental Quality (2006). • Alvarez, David A. "Chemical and Toxicologic Assessment of Organic Contaminants in Surface Water Using Passive Samples." Journal of Environmental Quality (2008). • Vanhaecke, Erwin. "Bioluminescence Assay for Measuring the Number of Bacteria Adhering to the Hydrocarbon Phase in the BATH Test." Applied and Environmental Microbiology (1988). • Frischer, M. E. "Bioluminescent Bacteria as Indicators of Chemical Contamination of Coastal Waters." Journal of Environmental Quality (2005). • Safra, Jacob E. "Bioluminescence." The New Encyclopedia Britannica. 15th ed. • "Bioluminescence." McGraw-Hill Encyclopedia of Science and Technology. 10th ed. 2007.

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