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Self-Assembly of Binary Phospholipids Mixtures

Self-Assembly of Binary Phospholipids Mixtures. Yellow Group: Peter Bowerman Andrea Hamill Ashish Jha Theresa LaFollette Tiffany Wilson Chaofang Yue Summer School June 2006. Experimental Background. Long and short tail lipids dissolved in solution

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Self-Assembly of Binary Phospholipids Mixtures

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  1. Self-Assembly of Binary Phospholipids Mixtures Yellow Group: Peter Bowerman Andrea Hamill Ashish Jha Theresa LaFollette Tiffany Wilson Chaofang Yue Summer School June 2006

  2. Experimental Background • Long and short tail lipids dissolved in solution • Useful in aligning proteins in solution to aid in structural NMR characterization • SANS can be used to characterize the structures formed by lipid mixture at low temperature

  3. Experiment Objectives • To determine the average dimensions of the self-assembled aggregate • derived from a Guinier analysis of the scattering at low Q • Determine the shape of the self-assembled aggregate • analyzing the scattering curves with different models • Determine the internal structure of the self-assembled aggregate • using selective deuteration in order to highlight only one lipid in the lipid mixture • Characterize the morphology of the lipid mixture • fitting simultaneously scattering curves from mixtures of hydrogenated and deuterated lipids

  4. Experiment Details DMPC = C36H72NO8P DHPC = C20H40NO8P

  5. Experiment Details • Samples (ratio DMPC/DHPC is 3 to 1) • 5% DMPC/DHPC in D2O • 5% d-DMPC/DHPC in D2O • 2% DMPC/DHPC in D2O • 5% DMPC/DHPC in D2O with NaCl • The 2% samples phase separated, no analysis • Distance(1m, 4.5m, 15.3m) • Measurements • Neutrons scattered by sample • Transmission (sample dependent) • Empty Cell (distance and sample dependent) • Blocked Beam (distance and sample dependent)

  6. Instrument- NG-7 SANS

  7. Instrument Characteristics

  8. Data

  9. Reasoning – Different Shapes

  10. Core-Shell Cylinder

  11. Results - Hydrogenated

  12. Global AnalysisPolydispersed Core-Shell Cylinder

  13. Results - Deuterated

  14. Final Model 56 A 53 A 12 A See physical model for better visualization

  15. Conclusion • The mixture forms a bicelle shape common among surfactants with different tail lengths • For NG-7 SANS testing information, refer to the website website

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