METABOLOMICS 2006

1. H O O H O H C H 3 O O C H O H 3 O H O O O O H O O H O H O H O H C H 3 O H O + O H O O O O O O O H O O O H O C O H O H O O O O H O O O O H O O H O O O O O O H O H O C H O H O H O H O O H O O H C 3 O H O S C S O 3 O H N O H O O H O O H S O H O METABOLOMICS 2006 polar lipids anthocyanins H 3 O H 3 glucosinolates H amino acids flavonols saccharides raffinose sucrose lactose Hex2Glycerol 0 min 90 Arabidopsis thaliana leaf extract HILIC-ESI-MS with a PolyHYDROXYETHYL A capillary 3 µm, 100 Å, 150x0.32 mm Gradient: Decreasing ACN (Courtesy of V.V. Tolstikov, Max Planck Institute-Potsdam)

2. 33,25 100 90 80 6,96 26,63 70 Relative Abundance 19,63 60 50 29,31 40 30 34,03 74,58 61,39 37,69 20 6,24 85,92 41,22 43,65 77,58 8,26 10 22,53 47,81 17,95 54,04 11,62 72,02 62,62 1,25 85,02 0 0 5 10 15 20 25 30 35 40 45 50 55 60 65 70 75 80 85 90 Time (min) 64,58 100 90 80 70 Relative Abundance 68,42 60 42,39 50 82,23 22,93 40 81,97 61,92 30 22,27 39,47 20 52,12 79,00 39,05 44,71 21,47 83,40 33,95 10 47,64 28,18 2,22 86,27 15,64 3,68 12,64 52,99 72,04 0 0 5 10 15 20 25 30 35 40 45 50 55 60 65 70 75 80 85 90 Time (min) Pipecolic acid 100 50 6.82 0 6.81 100 Lysine Relative Abundance 50 0 8.26 100 Adipicacid 50 0 20.27 100 Saccharopine 50 0 2 4 6 8 10 12 14 16 18 20 22 24 Time (min) THE WORST RETAINED SOLUTES IN REVERSED-PHASE ARE THE BEST-RETAINED IN HILIC! Sample: Arabidopsis thaliana leaf extract HILIC mode: Capillary of PolyHYDROXYETHYL A RPC mode: Capillary of C-18 Hypersil (Courtesy of V. V. Tolstikov, Max Planck Institute-Potsdam) * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * ** 5.70 HILIC-MS/MS: Isocratic analysis of individual compounds from serum or crude extracts in minutes! Sample: Crude soybean extract on PolyHYDROXYETHYL A column (Courtesy of Robert Croes, DuPont Biotech)