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Metabolomics

Metabolomics. Genomics – 25,000 Genes. DNA. RNA. Transcriptomics – 100,000 Transcripts. Proteomics – 1,000,000 Proteins. Protein. Biochemicals (Metabolites). Metabolomics – 2,800 Compounds. Systems Roles. Material. DNA. The ultimate potential of a cell. Information. RNA.

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Metabolomics

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  1. Metabolomics Genomics – 25,000Genes DNA RNA Transcriptomics – 100,000Transcripts Proteomics – 1,000,000Proteins Protein Biochemicals (Metabolites) Metabolomics – 2,800 Compounds

  2. Systems Roles Material DNA The ultimate potential of a cell Information RNA The current direction of a cell The functional capabilities of a cell Proteins Metabolites The limiting currency of a cell

  3. Mass Distribution of Compounds in the Human Metabolome • Metabolome • natively biosynthesized • monomeric • Complex metabolites • Xenobiome Number of cmpds per 20 daltons Mass

  4. Metabolomics is: • Precise in Nomenclature & Role • Quantitative & Statistically Approachable • Pharmaceutically Relevant • Less Complex • “Integromics”

  5. The birth of a science?

  6. 2003 NIH Roadmap

  7. FDA – Critical Path (2004) • DOE – DOE Genomics:GTL ROADMAP (2005)

  8. Orthomolecular medicine ‘Orthomolecular diagnosis is the process of determining the concentration of various substances in the body. Most of our experiments on orthomolecular diagnosis have involved the use of gas-liquid chromatography, ion exchange chromatography, or mass spectrometry. In this paper we discuss the approach to this problem that we have developed during the past five years.’ Clin. Chem. 20, 961 - 965 (1974).

  9. The birth of a science?

  10. Characteristics • Chemocentric platform • Unbiased analyses • Library based compound identification • Quantitative • Robust • Reproducible • CVs < 10%

  11. MS platforms Software Data Extraction -peak identification -peak alignment -peak deconvolution Chemical Identification -reference databases -ion spectra -grouping related ions -compound id Quantitation Quality Control Data Reduction Data Set MS/+ LC Sample Preparation MS/- GC MS/ei LIMS Preparation Analysis Informatics

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