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CHMI 2227E Biochemistry I

CHMI 2227E Biochemistry I. Enzymes: Regulation. Regulation of enzyme activity. Regulation of enzyme activity 1. Allostery. ATCase. Carbomoyl phosphate. Asp. Regulation of enzyme activity 1. Allostery. Regulation of enzyme activity 1. Allostery - ATCase. CTP. ATCase.

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CHMI 2227E Biochemistry I

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  1. CHMI 2227EBiochemistry I Enzymes: Regulation CHMI 2227 - E.R. Gauthier, Ph.D.

  2. Regulation of enzyme activity CHMI 2227 - E.R. Gauthier, Ph.D.

  3. Regulation of enzyme activity1. Allostery CHMI 2227 - E.R. Gauthier, Ph.D.

  4. ATCase Carbomoyl phosphate Asp Regulation of enzyme activity1. Allostery CHMI 2227 - E.R. Gauthier, Ph.D.

  5. Regulation of enzyme activity1. Allostery - ATCase CHMI 2227 - E.R. Gauthier, Ph.D.

  6. CTP ATCase Regulation of enzyme activity1. Allostery - ATCase CHMI 2227 - E.R. Gauthier, Ph.D.

  7. ATP Adenylate Cyclase cAMP cAMP Phosphodiesterase Caffeine AMP Regulation of enzyme activity2. Regulatory subunits CHMI 2227 - E.R. Gauthier, Ph.D.

  8. Regulation of enzyme activity3. Regulation by covalent modification CHMI 2227 - E.R. Gauthier, Ph.D.

  9. Regulation of enzyme activity3. Regulation by phosphorylation CHMI 2227 - E.R. Gauthier, Ph.D.

  10. Adrenaline receptor Adenylate cyclase Adrenaline Outside the cell Inside the cell G Protein ATP cAMP Glycogen (glucose stores) Protein Kinase A (inactive) Phosphorylase kinase Glycogen Phosphorylase-PO4 a a a g g b b ↑Intracellular glucose PKA-cAMP (active) ↑Energy Phosphorylase Kinase-PO4 Glycogen Phosphorylase Run Like Hell! Regulation of enzyme activity3. Phosphorylation  Adrenaline CHMI 2227 - E.R. Gauthier, Ph.D.

  11. Regulation of enzyme activity4. Regulation of enzyme stability CHMI 2227 - E.R. Gauthier, Ph.D.

  12. Ub E1 Ub Enzyme E1 E2 Ub E2 E3 E3 Ub Enzyme Ub Ub Ub Ub Regulation of enzyme activity4. Regulation of enzyme stability CHMI 2227 - E.R. Gauthier, Ph.D.

  13. Enzyme Ub Ub Ub Ub Ub Ub Ub Ub Proteasome Degraded enzyme Regulation of enzyme activity4. Regulation of enzyme stability CHMI 2227 - E.R. Gauthier, Ph.D.

  14. Regulation of enzyme activity5. Limited proteolysis CHMI 2227 - E.R. Gauthier, Ph.D.

  15. Regulation of enzyme activity5. Limited proteolysis CHMI 2227 - E.R. Gauthier, Ph.D.

  16. Small intestine Secreted by pancreas Regulation of enzyme activity5. Limited proteolysis CHMI 2227 - E.R. Gauthier, Ph.D.

  17. Regulation of enzyme activity5. Limited proteolysis CHMI 2227 - E.R. Gauthier, Ph.D.

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