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Garbelotto Lab 2009 Big Sur SOD Research

Garbelotto Lab 2009 Big Sur SOD Research. - SOD Spore Surveys & SOD Blitz / Outreach - Phosphonate Treatment of Tanoaks and CLOs - Long Term Efficacy of SOD Treatments - Resistance / Tolerance in Tanoaks Transmission of SOD Through Pruning. SOD Spore Monitoring.

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Garbelotto Lab 2009 Big Sur SOD Research

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  1. Garbelotto Lab 2009Big Sur SOD Research • - SOD Spore Surveys & SOD Blitz / Outreach - Phosphonate Treatment of Tanoaks and CLOs - Long Term Efficacy of SOD Treatments - Resistance / Tolerance in Tanoaks • Transmission of SOD Through Pruning

  2. SOD Spore Monitoring 8 Field Monitoring Sites 16 Buckets / Site 5 Leaves / Bucket = 640 leaves sampled every 3 weeks Starting again in February 08

  3. Air & Stream Sampling for SOD Spores

  4. Bay Laurel / Tanoak SOD Spore Survey Temp (C) Rain (mm) Date

  5. Spatial Autocorrelation Analysis of SOD Distribution Mascheretti, et. al. 2008 Molecular Ecology

  6. SOD Blitz – Disease Monitoring

  7. SOD Blitz / SOD Treatment Training Sessions

  8. Injection Treatment Topical Treatment

  9. Save the Tanoaks Project SOD Treatment Sites

  10. Long Term Efficacy of SOD Treatments

  11. Long Term Efficacy of SOD Treatments Agrifos vs. Azomite Treatments Azomite & Limewash Canker Size (% of Untreated Positive Control) Agrifos & PentraBark Novato Mature CLOs Detached Branch Assay

  12. Effect of Tree Size on Treatment Efficacy

  13. Tanoak Common Garden Experiment Shadehouse

  14. Tanoak Out Planting – Common Garden Experiment

  15. Tanoak Assays Whole Seedling Inoculation Tanoak Petiole Plug Assay Untreated Control Tree Infected Seedling Phosphonate Treated Tree

  16. Whole-seedling inoculation 9 8 7 6 Mean Lesion Length, Apr09 5 4 3 2 1 0 1 2 3 4 5 6 8 9 11 13 14 15 16 17 18 19 21 23 24 25 50 Family

  17. Transmission of SOD through pruning: ► Does tree pruning introduce SOD infection? ► Is the timing of cutting a factor? Test at: 1 week 1 month 2 months 4 months Pruning CLO Branches Zoospore Inoculator Grady Ranch Site

  18. Transmission of SOD Through Pruning

  19. Transmission of SOD Through Pruning Grady Ranch 11/27/07

  20. Zoospore Inoculator for P. Ramorum 1 cm Zoospores are in Direct Contact with Tree for 24 hrs. Grafting Wax Creates a Water Tight Seal

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