10 likes | 97 Views
Supplementary Fig. 1. a. s pot peptide A1-E19 K15-P 20aa, 1aa slide G1-K5 K15-M 20aa, 1aa slide M1-M21 triple dots each of: Btk (aa180-199) Btk (aa194-213) HIV-Nef (aa64-83) HIV-Nef (aa64-83, mut. T>R) NS5A (aa2313-2332, mut. C>S) NS5A (mut. p eptide 1)
E N D
Supplementary Fig. 1 a spot peptide A1-E19 K15-P 20aa, 1aa slide G1-K5 K15-M 20aa, 1aa slide M1-M21 triple dots each of: Btk (aa180-199) Btk (aa194-213) HIV-Nef (aa64-83) HIV-Nef (aa64-83, mut. T>R) NS5A (aa2313-2332, mut. C>S) NS5A (mut. peptide 1) NS5A (mut. peptide 2) M22-N9 K15-P truncation, triple dots N10-N27 K15-M truncation, triple dots N28-P27 K15-P alanine scan, 1 aa slide P28-R30 K15-M alanine scan,1 aa slide 20 20 20 25 25 25 30 30 30 20 25 30 15 15 15 15 10 10 10 10 5 5 5 5 1 1 1 1 A A K K M GST-Hck SH3, 1.25 µg/µl GST 1.25 µg/µl A A B B B B G G G H H H G K K H M M M N N N P P P Q Q Q N R R R P GST 1.25 µg/µl GST-Lyn SH3, 1.25 µg/µl Q R