MICROFLUIDICS

1. MICROFLUIDICS Division of Technology Transfer Licensing and Research Collaboration

2. Microfluidic Technologies Available for Licensing Micro-scale Handling System Sample Loading And Injection Microfluidic Device Small Volume Transport Electro-Osmotic Pump Subatmospheric Pressure Chamber Electro-Pneumatic Distributor

3. Microscale Fluid Handling System • A solution for conducting microscale reactions (digestion, separation etc.) and for efficiently transporting microliter to picoliter samples from a chip to an analytical device and/or a collection device. • Advantages • Efficient Sample Transport • Reduces manipulations (e.g.. flushing) • Reduces/eliminates problems of sample carryover • Savings • Less Sample, Reagent(s), Time

4. Microscale Fluid Handling System • The System • Multiple sample introduction methods • Pressure, electrokinetic Injection • Single or multiple channel(s) & channel designs • Parallel, circular/ cylindrical, trapezoidal • Multiple sample transfer methods • Droplet, spray, or stream • Multiple analytical or collection devices • ESI, MALDI, NMR, Fraction Collector, Chip, Multi-well Plate • Sample either liquid or gas • License non-exclusively - Currently 5 licensees

5. Sample Loading and Injection Device • A solution for a universal interface device for transferring samples in series or parallel from sample container (e.g. multi-well plates) into channels of a multi-channel microfluidic device and/or into an analytical device that can be integrated into or separated from a microchip. • Advantages • Uses standardized sample plates • Variable sample volumes • Reusable or disposable device

6. Sample Loading and Injection Device • The System • Sample introduction • Pressure, Electrokinetic, Vacuum, etc. • Sample trapped/digested inside loading channels • High hydrodynamic resistance • Sample Elution • Micro, analytical, and/or collection Device • Sample separation (optional) • Electrical potential, etc • License exclusively or non-exclusively

7. Microchip Integrated Open-Channel Electro-osmotic Pumping System • A solution to control fluid dynamics in microfluidic device by using pump(s) to generate electro-osmotic flow or pressurized flow in the device and/or to perform sample transfer, gradient generation or fraction collection/deposition. • Advantages • Easily integrated into existing microchips • Its fabrication ensures high manufacturing and operating reproducibility • Simple design

8. Microchip Integrated Open-Channel Electro-osmotic Pumping System • The System • Single or multiplexed pumps • The voltage drop for operation of the electro-osmotic micro pump may vary from a few tens to thousands of voltsdepending on the length of the pumping channels and desired flow rate and pressure. • License Exclusively or Non-exclusively

9. Electro-pneumatic Distributor for Multiplexed Myu-Tas Devices • The purpose of the distributor is to supply simultaneous electric current and pressurized gas to control individual channels of a microchip system in an assembly to use with electrospray mass spectrometry. • Advantages • Maximizes sample throughput for analyzing samples • Decreases time between sample analysis • Eliminates need for flushing of sample • Eliminates need for washing sample probe • Fast switching times • Eliminates/ reduces cross contamination • Decreases the number of runs • Well plate samples can be used for further studies

10. Electro-pneumatic Distributor for Multiplexed Myu-Tas Devices • The System • The distributor contains a gas channel and an electric conductor, which supplies an electric current and pressurized gas to the system. • The electrical current forces the sample to flow in a uniform direction, which controls sample flow dynamics. • The pressure created in the system controls fluid dynamics in electric field free regions. • Each sample container/well is connected by an independent microchannel distributor to separate electrospray tip. • License exclusively or non-exclusively

11. Small Volume Transport • A solution for moving small volumes of sample through the capillary channels or tubing of a microfluidic device, especially long distances. • Advantages • Minimal loss or dilution of sample. • Minimal cross contamination between samples • Minimal loss of sample to channel walls • Washes inserted between samples • Faster sample changes • Multiple sample plugs injected at closely spaced intervals • Samples can be transported long distances with high speed to devices, such as an NMR.

12. Small Volume Transport • The System • A sample/wash plug is formed between immiscible liquid plugs and immiscible liquid lining the transport channel walls. • System Characteristics • Immiscible carrier – e.g. fluorocarbon • Distances – yards • Channel walls - fluorine rich surface • Teflon (PTFE, ETFE, FEP, NGFP) • License exclusively or non-exclusively

13. Subatmospheric,Variable Pressure Delivery Chamber • A solution for more efficient sample transfer from electrophoresis capillary or microchip to a mass spectrometer through an electrospray chamber by controlling pressure to allow fine control of sample flow rate from electrospray needle. • Advantages • Minimal sample loss • Lower evaporation of droplets • Efficient desolvation • Minimal power supply source needed

14. Subatmospheric,Variable Pressure Delivery Chamber • The System • Ports for introducing gas into and withdrawing gas from the chamber. • Capillary tube(s) or microchip with a groove or channel extending into chamber to deliver samples. • Sample moves from the sample delivery device to an electrospray tip in the chamber. • Subatmospheric pressure directs the sample flow from the electrospray tip into an analytical/collective device • License exclusively or non-exclusively

15. Opportunities & Facilities for Microfluidics Research • Director – Barry Karger • Bioanalytical instrumentation capabilities • State of the art facilities and advanced methodologies for proteomics research and biomarker identification • http://www.barnett.neu.edu/

16. Mass Spectrometry: The Institute operates 16 interfaced mass spectrometers; these include: • 1 Thermo Electron LTQ-FT   Hybrid Linear Ion Trap-Fourier Transform MS • 1 Lab-built, High Throughput LC MALDI-TOF MS (2 kHz Laser).• 1 Applied Biosystems AB 4700 MALDI TOF-TOF MS • 1 Micromass QTOF1 QP TOF MS• 2 Agilent 5973 GC-MS• 1 Applied Biosystems Mariner ESI orthogonal extraction TOF-MS• 1 Applied Biosystems Voyager DESTR TOF-MS• 2 Thermo Electron LTQ Linear Ion Trap • 1 Thermo Electron LCQ Deca XP 3D ion trap MS• 3 Thermo Electron LCQ Classic 3D ion trap MS• 1 Thermo Electron TSQ 700 triple QP MS• 1 Thermo Electron TSQ 7000 triple QP MS• 1 SCIEX API III PLUS triple QP MS • 1 9-node computer cluster (18 CPU's) reported as of 4/05. II. Separation Instrumentation:This includes free-standing devices, listed in the following; other units are integrated both into mass spectrometers and NMR equipment. • Beckman Proteome Lab 2D LC System• Eksigent nanoLC System• 1 Advion Nanomate 100• 1 Bio-Rad 2D gel electrophoresis system • 2 Agilent 1100 HPLC systems• 2 Agilent 1100 Cap HPLC systems• 1 Agilent G 1602 CE system• 1 Amersham Biosciences MDLC system• 1 Amersham Biosciences AKTA FPLC • 5 Beckman CE systems• 1 Bischoff HPLC system• 3 Dionex Ultimate nanoLC systems• 2 Thermo Electron Surveyor LC sys • 4 Agilent  1090 HPLC's• 3 Agilent  1100 LC systems• 1 Shimadzu HPLC system• 2 Thermo Electron Surveyor LC sys III. NMR and LC-NMR:The James and Faith Waters 500 MHz NMR Facility 500 MHz NMR System (Varian Unity-Inova)• Conventional 5 mm NMR• LC NMR• High-Throughput Flow NMR• Microcoil NMR Opportunities & Facilities for Microfluidics Research

17. Opportunities & Facilities for Microfluidics Research • Center for Subsurface Sensing and Imaging Systems • Academic Partners are NU-lead, BU, RPI, and UPRM. • Strategic affiliates include MGH, Lawrence Livermore and Idaho Nat’l Labs, Woods Hole, and Sloan-Kettering Cancer Ctr. • Industrial partners include Raytheon, ADI, Textron, Lockheed Martin, Cardiomag Imaging, Mercury, Transtech, GSSI, and Siemens. • Director - Michael Silevitch

18. Opportunities & Facilities for Microfluidics Research • Advanced optical instrumentation for microscopic characterization • Software algorithms for microfluidic analysis systems • http://www.censsis.neu.edu/ • $20 million from The Gordon Foundation

19. Opportunities & Facilities for Microfluidics Research CANECS CANECS Center for advanced nanomaterials for energy conversion and storage • LEAP – Laboratory for electrochemical advanced power • Fuel Cell Concept Laboratories • Director – Sanjeev Mukerjee • Advanced electrocatalyst capabilities for proton exchange membrane systems • Micro fuel cell concept characterization and evaluation • http://www.chem.neu.edu/web/faculty/mukerjee.html

20. Opportunities & Facilities for Microfluidics Research • Center for High-rate Nanomanufacturing • Academic Partners • Northeastern University – Lead, U. Mass-Lowell, U New Hampshire, Michigan State U., Museum of Science • Director – Ahmed Busnaina • http://www.nano.neu.edu /index.html

21. Opportunities & Facilities for Microfluidics Research • Center for High-rate Nanomanufacturing • Kostas Facility for Micro- & Nano-Fabrication of Microfluidic Devices • A core facility for the NSF Center for High Rate Nanomanufacturing • Five thousand feet of Class 10, 1000 and 10000 cleanroom facilities • Capabilities for lithography, nanolithography, thin film deposition, wet chemical processes, etching, milling and characterization • Area for undergraduate and graduate student teams working on projects with corporate partners

22. DIVISION of TECHNOLOGY TRANSFER STAFF Volunteers, Coop MBA Students, Consultants (617) 373-8810