Catecholamines (dopamine [DA], norepinephrine [NE], epinephrine [EPI])

1. Catecholamines(dopamine [DA], norepinephrine [NE], epinephrine [EPI]) JonyMallik M.Pharm

2. Biosynthesis of Catecholamines

4. Important fetures of catecholamine biosynthesis, uptake and signaling • Biosynthesis • Release • Uptake (transporter) • Receptor-mediated signaling • Catabolism

5. Tyrosine hydrogenase: rate-limiting enzyme • TH is a homotetramer, each subunit has m.w. of 60,000 • Catalyzes –OH group to meta position of tyrosine • Km = M range; saturation under normal condition • Cofactor: biopterin; competitive inhibitor: -methyl-p-tyrosine • Sequence homology: phenylalanine hydroxylase and tryptophan hydroxylase • Phosphorylation at N-terminal sites:

6. Phosphorylation sites of Tyrosine Hydroxylase

7. Modulation of catecholamine synthesis • Neuronal activity increase would enhance the amount of TH and DBH at both mRNA and protein levels • TH is modulated by end-product inhibition (catecholamine competes with pterin cofactor) • Depolarization would activate TH activity • Activation of TH involves reversible phosphorylation (PKA, PKC, CaMKs and cdk-like kinase)

8. Dopa decarboxylase • Cofactor: pyridoxine; low Km but high Vmax • Also decarboxylate 5-HTP and other aromatic a.a.:aromatic amino acid decarboxylase (AAAD) • Inhibitor: -methyldopa Dopamine -hydroxylase • Cofactor: ascorbate; substrate: dopamine • Inhibitor: diethyldithiocarbamate (copper chelator) • DBH is a tetrameric glycoprotein (77kDa and 73kDa) • Store in the synaptic vesicle and releasable Phenylethanolamine N-methyltransferase (PNMT) Substrate: S-adenosylmethionine; regulated by corticosteroids

9. Catecholamines packed into the synaptic vesicles VMAT2: Non-selective and has high affinity to reserpine

10. Metabolism of dopamine • Major acidic metabolites: • 3,4-dihydroxy phenylacetic acid (DOPAC) • Homovallic acid (HVA)

11. Inactivation of Norepinephrine

12. Monoamine oxidase (MAO) • Cofactor: flavin; located on the outer membrane of mitochondria • Convert amine into aldehyde (followed by aldehydedehydrogenase to acids or aldehyde reductase to glycol) • MAO-A: NE and 5-HT (inhibitor: clorgyline); MAO-B: phenylethylamines (DA) (inhibitor: deprenyl) • Patient treated for depression or hypertension with MAO inhibitors: severe hypertension after food taken with high amounts of tyramine (cheese effect) Catechol-O-methyltransferase (COMT) • Enzyme can metabolize both intra- or extracellularly • Requires Mg2+ and substrate of S-adenosylmethionine

13. Uptake of catecholamines: transporter

14. Uptake transporters • Released catecholamines will be up-take back into presynaptic terminals (DAT, NET) • Transporter is a Na+ and Cl+-dependent process (ouabain [Na,K-ATPase inhibitor] and veratridine [Na channel open] block uptake process)

15. 3. Transporter is saturable, obeys Michaelis-Menten kinetics 4. 12 transmemebrane domain: intracellular phosphorylation and extracellular glycosylation 5. Uptake is energy dependent; can be blocked by tricyclic antidepressents, cocaine, amphetamine and MPTP

16. Regulation of DAT by various protein kinases

17. Localization of catecholamine neurons • Immunocytochemistry (ICH): antibody against synthesis enzyme, uptake transporter and receptor • In situ hybridization (ISH): cDNA or cRNA probe synthesis enzyme, transporter and receptor • Receptor autoradiography: radiolabelled ligand ([3H] or [125I]) against receptor

18. Noradrenergic projection (dorsal and ventral bundle) Cortex and hippocampus Dorsal bundle Spinal cord cerebellum (Locus ceruleus) Hypothalamus and Brainstem Ventral bundle

19. Dopamine projections (nigrostriatal, mesocortical, tuberohypophysial) Nigrostriatal projection Substantia nigra to caudate/putamen n. Tuberohypophysial projection Mesocotical projection Ventral tegmental area to nucleus accumbens and frontal cortex Hypothalamus to median eminence

20. Catecholamine receptors • Postsynaptic receptors locate on dendrites or cell body, axons or nerve terminals • Presynaptic autoreceptors locate on the same neuron: • a. terminal autoreceptor: control release • b. somatodendritic autoreceptor: synthesis control • c. major autoreceptor type: 2-adrenergic receptor in PNS/CNS; D2-dopamine receptor • d. exception: -adrenergic receptor facilitates NE release

21. Autoreceptor: inhibit transmitter release

22. Classification of Dopamine receptors

23. Feature of Dopamine receptors • Two subtypes of dopamine receptor: D-1 (short i3, long C-terminal) and D-2 like (long i3, short C-terminal) receptors • D2 receptors contain splicing isoform: D2L and D2S (87 bp) • D3 receptor has high affinity to atypical neuroleptics; D4 receptor bind tightly with clozapine • Chronic antagonist treatment up-regulate D2 receptors; agonist treatment might down-regulate the D2 receptor • Pharmacological application: anti-Parkinson (D2 agonist), anti-psychotic (D2 antagonist), addictive drugs (DA transporter)

24. 2-D structure of dopamine D2 receptor

25. Classification of Adrenergic receptors

27. Features of Adrenergic receptors • Both NE and epinephrine bind to  and  receptors • 1 locates mainly in the heart and cortex; 2 predominate in the lung and cerebellum; 3 in the adipose tissue (significance in obesity) • -receptor stimulates AC; in turn, inactivates receptor via ARK and -arrestin • 1 is a post-synaptic receptor (three subtypes: 1A, 1B and 1D); while 2 is both post- and pre-synaptic receptor (three subtypes: 2A, 2B and 2C) • Representative ligands: propranolol ( antagonist),yohimbine ( agonist)

28. propanolol yohimbine

29. GPCR-mediated signal and internalization

30. Dynamics of catecholamine receptors (up-regulation and down-regulation) agonist antagonist catecholamine receptor