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Solid-state Fermentation by using DFM(Direct-fed Microorganism) for Feed & Feed-additives

Solid-state Fermentation by using DFM(Direct-fed Microorganism) for Feed & Feed-additives. Application of Solid-state Fermentation. Foods: Functional-foods and Food-ingredients Livestock: Probiotics Agricultural Area: Bio-fertilizer Pharmaceuticals: Functional Bio-active Ingredients.

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Solid-state Fermentation by using DFM(Direct-fed Microorganism) for Feed & Feed-additives

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  1. Solid-state Fermentation by using DFM(Direct-fed Microorganism) for Feed & Feed-additives

  2. Application of Solid-state Fermentation • Foods: Functional-foods and Food-ingredients • Livestock: Probiotics • Agricultural Area: Bio-fertilizer • Pharmaceuticals: Functional Bio-active Ingredients

  3. Microbial Fermentation • Method • Liquid-state Fermentation: Alcohol Production • Solid-state Fermentation: Fermented Food • Controlling Parameters • Chemical Factors: Nutrients, Growth Factors, etc • Physical Factors: Temp., pH, Oxygen, etc • Biological Factors:

  4. Solid-state Fermentation • Advantage • Less contamination by low water content • Oxygen-supply, CO2-removal and temp-control by aeration • Disadvantage • Difficulty in Mixing: Low in homogeneousness • Difficulty in temp-control by low conductivity • Difficulty in On-line monitoring • Experience-dependent

  5. DFM(Probiotics) in Livestock • Direct-fed Microorganism • Probiotics • Definition: Microbial cell preparations or components of microbial cells that have a beneficial effect on the health and well-being of the host.

  6. Design of Solid-state Fermentor Exhaust Line & Evaporation Door for Raw Materials-Supply & Inspection Liquid-supply Spray Nozzle Paddle-type Mixing Device Recovery for Final Products Aeration Holes: Temp-controlled Air-Supply through chamber Forced air-supply by Blower With Temp-controlled Air

  7. Overall Fermentation Processes Preparatory Stage Main Fermentation Manufacturing Stage Raw Materials: Feedstuff or Fermentation media Control Temp, Aeration & Mixing Microbial Growth Water Content Control: Water content Temp & Mixing DFM(Probiotics) Packaging Two-stage Fermentation 1st Stage: Aerobic Process 2nd Stage: Anaerobic Process TMF Water Content Functioanl Ingredients

  8. Solid-state Fermentation Process • Preparatory Stage • Media as Raw Materials: Feedstuff • DMF(Probiotics): High-density Microbial Cells • Functional Ingredients: Growth factors • 1st Stage: Aerobic Fermentation • Microbial Growth (Proliferation) & Enzyme Production • Providing Aerobic Condition: Air supply through aeration holes at the bottom of fermentor Continuous agitation (mixing) for aeration and mixing • 2nd Stage: Anaerobic Fermentation • Stabilization of grown-microorganisms • Producing Bio-active Microbial Metabolites & Flavours • Simultaneous providing anaerobic condition: Air supply through top of fermentor according to temp-control Continuous agitation (mixing) for aeration and mixing

  9. DFM(Probiotics) for Fermentation • Effective Microorganisms used for Solid-state Fermentation • Lactobacillus species • Bacillus species • Aspergillus species • Yeast (Saccharomyces) species

  10. TMF (Total Mixed Fermentation) • TMR (Total Mixed Ration) • Feedstuffs for Dairy Cow • Simple Mixing, None-Fermentation • TMF: Advanced TMR • Fermented Feedstuffs • Fermented DMF(Probiotics) • Composition: Live Probiotics: Effective Live-microorganisms Microbial components: Bio-active Microbial Products & Metabolites Microbial Enzymes: Various Enzymes

  11. Function of TMF in Livestock • Normalization of Microflora • Protection of inflammation and diarrhea • Prevention of various diseases • Enhancement in immunity and feed efficiency • Minimizing undigested materials • Providing various bio-active compounds TMF In Vivo • Minimizing fiscal smell and water content • Effective for Waste-treatment • Providing Well-being Environment • Reduction in insect plague In Vitro

  12. Equipments for DFM(Probiotics) Refrigerator for Stock and media Clean bench For aseptic work Shaking Incubator for seed culture Lab-scale & Pilot-scale Fermentor Incubator for microbial growth

  13. Equipments for TMF Supply-Device For Raw Materials Screw Conveyor Dumping Transfer Devices For TMF(Product) Screw Conveyor Solid-state Fermentor Liquid-supply Devices For DFM & Liquids FRP Tank & Feeding Pump Packing Device For Manufacturing Packing & Wraping

  14. Solid-state Fermentor (I) • Aeration Unit • Ring blower with Line heater • Dual way for aeration: Top & Bottom of Fermentor • Controlled by PLC (Temp & Process) • Agitation (mixing) Unit • Paddle-type Impeller • Mixing & Holding • Controlled by PLC (Temp, Aeration & Process) • Others • Liquid-supply Unit: • Special Screw Unit: Pushing-out final product

  15. Solid-state Fermentor (II) • Control Unit • PLC control with Programmed Software • Interlocked with Aeration-Temp-Agitation-Process Time • Data-storage and Ethernet Communication • User-friendly Interface • Consultation • Support by Highly Experienced & Educated Expertise • Experiences in Manufacturing & Process Control • Consultation in Design, Process & Manufacturing, Products • A/S & On-site Support

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