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Tumor-stromal Interactions in Metastatic B rain C ancer

Tumor-stromal Interactions in Metastatic B rain C ancer. Kathryn Suttling Mentor : Victor Blanco PI: Xiaoyang Qi, PhD Lab Department: Internal Medicine/ Hematology and Oncology Vontz Center for Molecular Studies. Our Model. Intracarotid Artery Injection. 3-4 Weeks.

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Tumor-stromal Interactions in Metastatic B rain C ancer

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  1. Tumor-stromal Interactions in Metastatic Brain Cancer Kathryn Suttling Mentor: Victor Blanco PI: Xiaoyang Qi, PhD Lab Department: Internal Medicine/ Hematology and Oncology Vontz Center for Molecular Studies

  2. Our Model Intracarotid Artery Injection 3-4 Weeks Dissection and Sectioning of the mouse brain Brain Slides Nude Mouse model Skin Cancer Cells 2ry AB-FITC (anti-rabbit) 2ry AB-TRITC (anti-mouse) 1st AB (Rabbit) against Ki-67 1ry AB (Rat) against CD11b Immunofluorescence CD11b Ki-67

  3. Histology of Experimental Melanoma Brain Metastases (H&E) H & E: Routine histopathological characterization to identify tumor cells. 10X 10X • 4X Normal- 10X 40X

  4. Stromal Cells in Melanoma Brain MetastasesImmunofluorescence DAPI: Marks nuclei (DNA). Dense areas of cells compared to different regions are generally tumorous. GFAP: High expression indicates reactive astrocytes. Nestin: Stem cell marker- Co-option of host cells. DAPI Merge GFAP DAPI/Nestin 4X 40X 40X Normal 4X

  5. Immune Cells in Melanoma Brain Metastases CD11b: Labels macrophages/microglia (Tumor Associated Macrophages) Ki-67: Labels proliferating cells Versican:Chondroitin sulfate; matrix component; possible immune activator 4X DAPI CD11b Merge Ki-67 Merge Versican DAPI 4X 4X

  6. Summary • Valuable model to study the progression and interaction of brain metastases of melanoma and stromal and immune cells. • Through immunofluorescence techniques, we identified astrogliosis, tumor’s co-option of host cells and immune cell involvement in melanocytic brain metastases. • Next steps are to figure out how to make tumor cells to uptake SapC-DOPS (anticancer liposomes). Right now there is targeting, but only around the tumors. • In the future, we will be looking for the potential therapeutic applications of SapC-DOPS

  7. My Experience • What have I learned? • Research process: • Trial and error • Experiments must be validated • Research takes time • About Myself: • In order to be good at something, work and time must be put into it. • Why does it matter? • To be good in something, you need to put in the work • Passion

  8. My Experience • What now? • Academic: Use basic lab techniques in future science classes. Gain an understanding of what is happening when an experiment is performed. • Professional: As I look towards graduate school, the option of engaging research will be available to me if I so choose. • Personal: This has taking me one step closer to figuring out what direction I want to take in life.

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