1 / 8

Recovery of the genetically selected broiler line

1) Department of Biochemistry and Molecular Biology, Penn State University, University Park, PA, 16802, USA. 2) Institute of Agriculture and Forestry, University of Tsukuba, Ibaraki 305-8572, Japan. 3) Department of Poultry Science.

naomi
Download Presentation

Recovery of the genetically selected broiler line

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. 1) Department of Biochemistry and Molecular Biology, Penn State University, University Park, PA, 16802, USA. 2) Institute of Agriculture and Forestry, University of Tsukuba, Ibaraki 305-8572, Japan. 3) Department of Poultry Science. Penn State University, University Park, PA, 16802, USA. 4) National Institute for Minamata Disease, Kumamoto 867-0008, Japan. Recovery of the genetically selected broiler line by transferring cryopreserved circulating PGCs. Atsushi Tajima 1) 2), Guy F. Barbato3), Palmer G. Cramer1), Carol H. Crouch 3) , Takashi Kuwana 4), Roy H. Hammerstedt 1) ,

  2. Materials and Methods 1 Fertilized Eggs (42L) Incubation (37.8 oC) Blood collection (Stage 13-15) Freezing Bicell (Nihon Freezer) 10% DMSO, -80 oC LN2

  3. Materials and Methods 2 Brown Leghon (BL) Eggs Dekalp, IL Thaw blood (Ice water 4 oC) Incubation (37.8 oC) Isolation of PGCs Remove DMSO Blood removal (stage 13-15) Injection Hatch Progeny testing

  4. Results Female BL(42L) Total number of chick hatched Number of white chick White chicks % Batch 1 4 4 100.0 2 2 1 50.0 3 5 3 60.0

  5. Total number of chick hatched Number of white chick White chicks % Batch Results Male BL(42L) 1 12 6 50.0 2 21 10 47.6

  6. Conclusion A broiler line (42L) was recovered using frozen/thawed circulating PGCs. Genetically important lines (grand parents, transgenics etc) can be recovered using frozen/thawed PCGs

  7. Acknowlegments Dr. Lary Vint (DeKalp) for providing Brown Leghorn eggs

  8. Financial support Ministry of Education, Science, Culture and Sports, Japan. Ministry of Environment, Japan.

More Related