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Review of Pilot data

Jennifer Layden, MD, PhD Assistant Professor of Medicine and Public Health Sciences Loyola University Health System. Review of Pilot data. Discuss 2 pilot data sets Review basic epidemiologic data Discuss ongoing / planned analyses. Hepatitis Pilot Data from two source populations.

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Review of Pilot data

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  1. Jennifer Layden, MD, PhDAssistant Professor of Medicine and Public Health SciencesLoyola University Health System

  2. Review of Pilot data • Discuss 2 pilot data sets • Review basic epidemiologic data • Discuss ongoing / planned analyses

  3. Hepatitis Pilot Data from two source populations Subjects enrolled in H3N study (B Tayo) N: 200 ff • Aims: • -chronic/recovered infection rates • -immediate cases/controls for: • -viral genotyping/sequencing • -host genotyping

  4. Data Analysis Plan • Stored samples run in LUMC reference lab • HCV: 4th generation Ab • HBV: Surface Ag • HIV: HIV-1/2 Ab • Aminotransferase level (ALT)

  5. METS CohortPilot Study(N=493)

  6. METS Pilot Study Comparison of HCV Antibody Positive to Negative Population Comparison of HBV S Antigen Positive to Negative Population

  7. Nigeria (H3N) Pilot Study(N=200)

  8. Nigeria Pilot Results Comparison of HCV Antibody Positive to Negative Population Comparison of HBV S Antigen Positive to Negative Population

  9. Logistic Regression:Association with HCV SeropositivityUnivariable Analyses

  10. Logistic Regression:Association with HBV S Ag SeropositivityUnivariable Analyses

  11. Summary of Basic Analysis • Overall higher than expected HCV Sero-positivity in Ghana and Nigeria • Low mean ALT levels • Overall low values • No variation seen by HCV status • Higher values in those with +S Ag • HCV Seropositivity associated with: • HIV + status (>6 OR’s) • HBV S Ag + associated with: • -higher ALT values

  12. Ongoing Analyses • Samples to CDC: • HCV / HBV viral sequencing / genotyping • Viral load measurements • Samples to Duke: • Genotyping of IL28B SNP’s • Association of cleared vs chronic infection • Overall allelic variation

  13. HBV S Antigen / S Gene Sequencing Concordancy Results • S Ag positive: 85 individuals • S Gene positive: 136 individuals

  14. Factors Associated HbSAg and S gene Status

  15. Questions Arising from these results • What contributes to the discordant results? • Is there a high level of occult HBV? • Significance? • Can there be mutations within the SAg causing the low rate?

  16. Current Progress of Pilot Data • CDC based testing: • HBV: Check HBV EAg and EAb, CoreAb, SAb and HBV DNA quant • HCV: Finishing sequencing results • Assess for chronic infection rates • Duke • IL28B genotyping • Allelic variation across SNPs

  17. Kumasi Blood Bank Protocol • Active Enrollment • Re-testing and survey administration to assess risk factors of acquistion

  18. Investigators • LUMC: N Mora, I Berzin, L Dugas, B Tayo, A Luke • METS: A Luke, PhD; J Plange-Rhule, MD, PhD • Nigeria: B Tayo, PhD, B Salako, MD, A Akere, MD • CDC: J Forbi, PhD, Y Khadanov, PhD • Duke: T Urban, PhD, PharmD

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