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Haitham A. Shaban Assistant Researcher, NRC Teaching Assistant , American University in Cairo

Radiobiology & Radiobiology of Accelerated Heavy Ions. Haitham A. Shaban Assistant Researcher, NRC Teaching Assistant , American University in Cairo. RADIOBIOLOGY : THE STUDY OF THE EFFECTS OF IONIZING RADIATION ON BIOLOGIC TISSUE. RADIATION EFFECT CELL THEORY.

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Haitham A. Shaban Assistant Researcher, NRC Teaching Assistant , American University in Cairo

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  1. Radiobiology & Radiobiology of Accelerated Heavy Ions Haitham A. ShabanAssistant Researcher, NRCTeaching Assistant, American University in Cairo

  2. RADIOBIOLOGY:THE STUDY OF THE EFFECTS OF IONIZING RADIATION ON BIOLOGIC TISSUE

  3. RADIATION EFFECT CELL THEORY Radiation interaction at the atomic level can produce molecular change, as a result, cell will be produced deficient.

  4. ATOMIC COMPOSITION OF BODY: 80% 25.7% 10.7% 2.4% 0.1% O.1% 0.8% 02 H2 C N2 P S TRACE ELEMENTS

  5. MOLECULAR COMPOSITION OF THE BODY 80% 15% 2% 1% 1% 1% WATER PROTEIN LIPIDS CARBOHYDRATES NUCLEIC ACID OTHER

  6. Radiation Biology

  7. CELL RESPONSE TO RADIATION LYNPHOCYTES SPERMATOGONIA OSTEOBLASTS SPERMATIDS MUSCLE CELL NERVE CELL RADIOSENSITIVE RADIORESISTANT

  8. RADIATION ENERGY TRANSFER DETERMINANTS LET RBE

  9. LET-LINEAR ENERGY TRANSFER THE AVERAGE ENERGY DEPOSITED PER UNIT LENGTH OF TRACK keV/ μm

  10. LET α X-RAYS

  11. LET GAMMA RAYS X-RAYS ALPHA PARTICLES IONS OF HEAVY NUCLEI CHARGED PARTICLES LOW ENERGY NEUTRONS LOW LET HIGH LET

  12. RBE –RELATIVE BIOLOGIC EFFECTIVENESS Relative capabilities of radiation with different LETs to produce particular biologic response. DOSE IN Gy FROM 250 KVP X-RAYS DOSE IN GRAY OF TEST RADIATION

  13. EFFECTS OF IONIZING RADIATION ON DNA BASE DAMAGE SINGLE STRAND BRAKE DOUBLE STRAND BRAKE CROSSLINKING

  14. Clustered DNA damages Fragment ofDNA

  15. LOSS OR CHANGE OF A BASE ON DNA RESULTS IN ALTERATION OF THE BASE SEQUENCE. SEQUENCE OF THE BASE STORES GENETIC INFO- SERIOUS CONSEQUENCE TO THE CELL CLASSIFIED AS: MUTATION BASE DAMAGE

  16. Radiation induced mutagenesis Guanine

  17. RELATIVELY INCONSEQUENTIAL IN TERMS OF CELL KILLING. WILL BE EFFICIENTLY REPAIRED , WITH LITTLE, IF ANY, LONG TERM CONSEQUENCES TO THE CELL SINGLE STRAND BRAKES

  18. VERY DIFFICULT FOR THE CELL TO BE REPAIRED. CAN LEAD TO CELL KILLING. IF REPAIR DOESN’T TAKE PLACE, THE DNA CHAINS CAN SEPARATE. SERIOUS CONSEQUENCE TO CELL LIFE. DOUBLE STRAND BRAKES

  19. CROSSLINKING

  20. Investigation of biological effects of accelerated heavy ions forms the “new radiobiology”

  21. The JINR accelerators Accelerator Particles Energy (up to) Lab Phasotron Protons 660MeV LNP U-200 Heavy ions 10MeV/amu LNR U-400M Heavy ions 50 MeV/amu LNR Sinchro phasotron Protons, Heavy ions 10 GeV/amu LHE Nuclotron Protons, Heavy ions 6 GeV/amu LPHE

  22. The nuclotron beams Intensity (particles per cycle) Beam Present status In 2009 p, d 1010 - 1011 1013 4He 109 1010 12C 109 7.109 24Mg 108 3.108 56Fe 106 107 84Kr 104 2.107 The maximal energy of particles up to 6 GeV/amu

  23. The dose distribution of radiation in matter 1 unit of the dose 1 unit of the dose X-rays Fe ion

  24. What radiobiological problems can be solved at use of the accelerated heavy particles?

  25. Heavy ions is apowerful tool for the solve of fundamental problems of radiation genetics

  26. block of DNA repair normal repair super fast repair Bacterial cells The RBE problem was solved at the Flerov Lab accelerators DNA repair capacityof the living cells determines the type of RBE on LET dependence

  27. LABORATORY OF RADIATION BIOLOGY Kinetics of DNA repair PAC 08

  28. 2 chromosomes №1 fragment 3D dimension of human chromosome Chromosome 1 of human lymphocyte nucleus in interphase with fragment after irradiation 1 GeV protons at synchropha-sotron. The 3 D dimensi-on picture was obtained by using FISH technique and confocal microscopy.

  29. Induction of mutagenic DNA repair by heavy ions luciferase FMNH2+ RCHO + O2 FMN + RCOOH +H2O + h

  30. -rays • Formation of stable chromosomal aberration after heavy ion irradiation of human cells Stable chromosomal aberration Successful of cell division

  31. Unstable chromosomal aberration • Formation of unstable chromosomal aberration after heavy ion irradiation of human cells Block of cell division

  32. Acknowledgment Thanks to ASRT - Egypt Thanks to ASRT - Egypt Thanks to ASRT - Egypt Thanks to JINR - Dubna Thanks to JINR - Dubna Thanks to JINR - Dubna Thanks to Prof. Е. А. Krasavin Thanks to Prof. Е. А. Krasavin

  33. Thank you for the attention 

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