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Mesenchymal precursor cells in the blood of normal individuals

Mesenchymal precursor cells in the blood of normal individuals. Nathan J Zvaifler, Lilla Marinova-Mutafchieva, Gill Adams, Christopher J Edwards, Jill Moss, Jan A Burger, and Ravinder N Maini (Arthritis Research 2000, 2:477-488). Presented by Victoria Backes. Purpose.

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Mesenchymal precursor cells in the blood of normal individuals

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  1. Mesenchymal precursor cells in the blood of normal individuals Nathan J Zvaifler, Lilla Marinova-Mutafchieva, Gill Adams, Christopher J Edwards, Jill Moss, Jan A Burger, and Ravinder N Maini (Arthritis Research 2000, 2:477-488) Presented by Victoria Backes

  2. Purpose • To find alternative/additional sources for mesenchymal precursors or stem cells that could potentially be used for tissue engineering and gene therapy

  3. Background • What are stem cells? • What are MSCs? • Pluripotent vs committed progenitor cells • Other areas of stem cell research

  4. Elutriation Cell-proliferation assay Immunohistochemistry Quantification of BMPCs Anti-BMPR antibodies Alkaline phosphatase activity of circulating BMPCs Stromal-cell-derived factor (SDF)-1 PT-PCR Results illustrated in SEM Methods

  5. Isolated Cells

  6. BMPC-rich elutriation

  7. Results form BMPC-rich elutriation

  8. Proliferation

  9. Antibody Staining

  10. Supplemented Cells

  11. AP Activity

  12. PT-PCR Analysis

  13. Lineage dispute CD34 status? Role of BMPs Role of BMPRs Isolation difficulty CD34+ cells BMP2 Identification of BMPCs Use of antibodies Progenitor & precursor B cells Presence of OCs Discussion

  14. Conclusion

  15. References • Gibbs W. 2001. Stem cells, without the hype. Scientific American 284: 16-17. • Mosca j, Hendricks K, Buyaner D, Sproul J, Chaung L, Majumdar M, Chopra R, Barry F, Murphy M, Thiede M and others. 2000. Mesenchymal stem cells as vehicles for gene delivery. Clin Orthop 1:S71-S90. • Vander A, Sherman J, Luciano D. 2001. Human Physiology. 8th ed. New York: McGraw. 800 p. • Zvaifler N, Mutafchieva L, Adams G, Edwards C, Moss J, Burger J, Maini R. 2000. Mesenchymal precursor cells in the blood of normal individuals. Arthritis Research 2:477-488.

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