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Les power point sont sur le site du Master BS, dans l’onglet de l’UE FMBS106

Les power point sont sur le site du Master BS, dans l’onglet de l’UE FMBS106 www.masterbs.univ-montp2.fr Guidage axonal - Développement du cerveau - Remodelage neuronal - Mémoire. 1) Attraction by the midline 2) Crossing the midline 3) Molecular changes before and after the crossing

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Les power point sont sur le site du Master BS, dans l’onglet de l’UE FMBS106

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  1. Les power point sont sur le site du Master BS, dans l’onglet de l’UE FMBS106 www.masterbs.univ-montp2.fr Guidage axonal - Développement du cerveau - Remodelage neuronal - Mémoire

  2. 1) Attraction by the midline 2) Crossing the midline 3) Molecular changes before and after the crossing 4) AC or PC ? (DRL/WNT5) 5) Fasciculation Book chapter Navigating intermediate targets: the nervous system midline Dickson and Zou in Cold Spring Harbor Perspect Biol 2010 (Neuronal guidance - the biology of brain wiring)

  3. Commissural axons at the midline in vertebrates and Drosophila

  4. Morphogens involved in neuronal guidance and their receptors

  5. Netrins, Slits and their receptors. Receptor for attraction (DCC) Receptors for repulsion (UNC5 for Netrins; Robo for Slits)

  6. Guidance-cue receptor signaling strategies.

  7. Mutipe guidance cues control the pathfinding of spinal cord commissural axons along the dorso-ventral axis

  8. Midline crossing mutants in Drosophila Fig. 5.39 in Development of the nervous system (book)

  9. Molecular cues controlling the pathway choices at the Drosophila midline: COMM regulates ROBO1 trafficking and midline crossing

  10. Molecular cues controlling the pathway choices at the vertebrate midline: the Slit/Robo and Sema3B, 3F/Neuropilin2 systems control midline guidance

  11. Drosophila ventral nerve cord A Robo code for lateral positioning Rodent spinal cord

  12. DRL/WNT5 in the Drosophila brain: Grillenzoni et al., Development 2007 Mamalian Ryks in the developing nervous system: Fradkin et al., Trends in Neurosciences 2010

  13. The mushroom bodies (MBs) in the adult Drosophila brain Rein et al., 2002 Current Biology

  14. A wild-type  neuron  vertical branch 10mm 10mm  horizontal branch midline Srausfeld et al., MRT 2003 10mm 10mm

  15. The drl Receptor Tyrosine Kinase Fradkin et al., TINS 2010

  16. DRLis not expressed in the MBs (FASII)

  17. DRLis not expressed in the MBs (FASII)

  18. DRL in the embryonic brain

  19. drl LOF mutation induces no apparent defects in the embryonic brain but do induce defects in the embryonic nerve cord

  20. drl is not expressed in the MB neurons but is required for the MB development Grillenzoni et al., Development 2007

  21. MARCM clones -/- -/- -/- +/- mutant phenotype mutant phenotype ? cell-autonomous ?

  22. -/- -/- mutant phenotype

  23. +/+ -/- +/+ +/- No mutant phenotype, therefore non cell-autonomous

  24. Wnt5-/Y; -GAL4 UAS-mGFP/+ Wnt5-/Y; -GAL4 UAS-mGFP/+; UAS-Wnt5/+ Conclusions: Wnt5 is necessary for the axonal growth in an intrinsic manner

  25. A growing b axon b axon WNT5 axonal growth ? Secreted protein Transmembrane receptor

  26. A stoping b axon extrinsic MB axon b axon WNT5 stop ? LIO RTK

  27. Structural features of Ryk subfamily receptors

  28. Cleavage and nuclear translocation of the Ryk intracellular domain (ICD) mediates neurogenesis

  29. Wnt/Ryk-mediated axon repulsion in the developing versus injured adult spinal cord

  30. Ephrins and their Eph receptors

  31. Wnt/Ryk-mediated axon repulsion counterbalances ephrinB1/EphB-mediated attraction in retinotopic mapping

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