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C. albicans yeast and hyphae

SDS-DTT extract. 50 mM Tris-HCl, 0.1 M EDTA, 2% SDS , 10 mM DTT at 100°C. NaOH extract. exochitinase extract. C. albicans yeast and hyphae. A. Cell grinding: 10 mM Tris-HCl, pH 7.4, 1 mM PMSF. Successive steps of washes/centrifugations:. Pellet (CW1). 1 - water. 2 - 85 mM NaCl.

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C. albicans yeast and hyphae

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  1. SDS-DTT extract 50 mM Tris-HCl, 0.1 M EDTA, 2% SDS, 10 mM DTT at 100°C NaOH extract exochitinase extract C. albicans yeast and hyphae A Cell grinding: 10 mM Tris-HCl, pH 7.4, 1 mM PMSF Successive steps of washes/centrifugations: Pellet (CW1) 1 - water 2 - 85 mM NaCl 3 - 34 mM NaCl Pellet (CW2) 4 - 17 mM NaCl Pellet (CW3) β-1,3-glucanase 30 mM NaOH Pellet (CW4) exochitinase β-1,3- glucanase extract B 20 outside Number of proteins intracellular not predictable 10 SDS-DTT extract NaOH extract β-1,3-glucanase extract exochitinase extract

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