Running an experiment in a completely contained environment as oppose to 2 Liter plastic

1. Discussion • A One-Sample T-Test revealed that there was no significant difference between the CO2, pH and Spectrophotometer measurement between both Thalassiosirapseudonana and Phaeodactylumtricornutum • In Thalassiosirapseudonana, although the spectrophotometer found more growth in concentration with Ferrous Sulfate as well as a more acidic pH, which indicate the absorption of CO2. • In Phaeodactylumtricornutum, all data expressed showed that there was more growth in the control rather than the culture ran with Ferrous Sulfate. • The results of this experiment differ from the work of Coale and Martin as Ferrous Sulfate has no significant effect on the growth of diatoms. Picture 7: Stock cultures of algae maintained for new trials or incase of experimental mishaps Conclusion • In Conclusion, the results of this experiment differed from the work of Coale and Martin and their idea in exponentially growing diatoms with Ferrous Sulfate. Picture 8: In Trial 2 of data collection, the vessel in growing plankton was altered to a water dispensing jug in an attempt to increase sterility. Picture 9 and 10: The Autoclave machine served as a means of sterilizing certain substances such as media and glassware Future Studies • Running an experiment in a completely contained environment as oppose to 2 Liter plastic • Determine if the pH change that occurs because of T. pseudonana and P. tricornutum has an effect on other organisms living in the same environment • Observing the effects of other species of Diatoms and their growth with Ferrous Sulfate Picture 10: The spectrophotometer was used to measure growth in T. pseudonana and P. tricornutum. The wavelengths used were 660nm and 750nm respectively. Bibliography • Ackerman, Suzanne. "Human-Related Sources and Sinks of Carbon Dioxide." Climate Change - Greenhouse Gas Emissions. 9 Sept. 2008. 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