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Application of Hydrogels in Electrospun Arterial Scaffolds

Application of Hydrogels in Electrospun Arterial Scaffolds. Patrick K. Barranger. Need Biodegradable Vascular Grafts. Coronary heart disease remains the #1 cause of death in the U.S. Atherosclerosis – characterized by thickening of arterial wall

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Application of Hydrogels in Electrospun Arterial Scaffolds

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  1. Application of Hydrogels in Electrospun Arterial Scaffolds Patrick K. Barranger

  2. Need Biodegradable Vascular Grafts • Coronary heart disease remains the #1 cause of death in the U.S. • Atherosclerosis – characterized by thickening of arterial wall • Autologous replacements not always the viable option • Current options on the market not sufficient • ePTFE • Dacron®

  3. Vascular Architecture • Intima • Single layer of ECs • Contains mostly collagen • Media • Thickest layer consists of SMCs, elastic laminae, collagen, elastin fibrils • Adventitia • Dense fibroelastic connective tissue containing FBs

  4. Electrospun Scaffolds

  5. PCL PCL r = d r = c r = b r = a Hydrogels • Water Swollen Polymers • Protein Incorporation • Collagen • Bovine Serum Albumin • Gelatin • Cell Infiltration • Low Stiffness • Future Growth Factor Incorporation • Easy to Make • Low Cost

  6. Step 1: Establish Protocols • Developed Protocols • Alginate Hydrogels • Protein Incorporation • Collagen • Bovine Serum Albumin • Gelatin • Scaffold Application

  7. Step 2: Cellular Studies • 10 Day Study • Static and Encapsulation • BSA • Gelatin • Collagen • 1% Alginate • DAPI Stained • Histology • Experimental DPA Assay

  8. DAPI Stains: Encapsulation

  9. DAPI Stain: Static Seeding

  10. Histology : Encapsulation

  11. Histology: Static Seeding

  12. Experimental DPA Assay

  13. 3 Layer Prototype

  14. Step 3: Mechanical Testing • Tested: • Burst Strength • Compliance

  15. Future Studies • Limit de-lamination through new techniques • Quantify the benefits of protein incorporation • Effects of Genipin and EDC • Increase cell density • Sodium Bicarbonate incorporation

  16. Q & A

  17. Thank You! • Michael McClure • Dr. Bowlin and Lab • VCU BBSI Program

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