1 / 13

Title:

Title: Genetic analysis of Soil-borne cereal mosaic virus response in durum wheat: Evidence for the role of the major QTL QSbm.ubo-2BS and of minor QTLs. Journal: Molecular Breeding Author’s names:

niran
Download Presentation

Title:

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Title: Genetic analysis of Soil-borne cereal mosaic virus response in durum wheat: Evidence for the role of the major QTL QSbm.ubo-2BS and of minor QTLs. Journal: Molecular Breeding Author’s names: Marco Maccaferri1, Rossella Francia1, Claudio Ratti1, Concepcion Rubies-Autonell1, Chiara Colalongo1, Gianluca Ferrazzano2, Roberto Tuberosa1, Maria Corinna Sanguineti1 Affiliations and addresses: 1 Department of Agroenvironmental Science and Technology, University of Bologna, Viale G. Fanin 44, 40127 Bologna, Italy 2 Società Produttori Sementi Bologna S.p.A., Divisione Ricerca, Via Macero 1, 40050 Argelato (BO), Italy corresponding author: marco.maccaferri@unibo.it Phone: +39 051 2096645 Fax: +39 051 2096241

  2. Supplementary material Figure 1. Frequency distributionsof the phenotypic traits recorded for the Simeto ×Levante (SxL) recombinant inbred line population (180 F6:7 RILs) in two field trials carried out in Cadriano (44°35’N 11°27’E), Northern Italy (Po valley), under high SBCMV-disease pressure during two seasons (2008 and 2009). • The following SBCMV-response traits were measured at the plant growth stages showing the maximum disease symptom intensity, i.e. from mid-end of tillering (Z25 in the Zadoks scale, Zadoks et al., 1974) to first (Z31) and second (Z32) node appearance stages (corresponding to a period from the end of February to April): • symptom severity (SS), visually scored following a 0-4 scale at three and five scoring/sampling dates in 2008 and 2009, respectively; • leaf virus concentration (ELISA), determined using DAS-ELISA (reported as absorbance units at 405 nm) on samples collected at two scoring/sampling dates in both 2008 and 2009. • Frequency distributions were obtained using the mean values across scoring/sampling dates of each year for SS, ELISA values, leaf greenness (SPAD) and Normalized Difference Vegetation Index (NDVI). Grain yield (GY) was also considered. • Mean values for the RILs (S×L), Simeto (S), Levante (L) and for the highly susceptible check cv. Grazia (G) are indicated with arrows and reported together with the corresponding standard deviation in parenthesis.

  3. G (3.42 ± 0.29) L (0.13 ± 0.10) S (2.78 ± 0.17) G (1.41 ± 0.16) L (0.02 ± 0.01) S (1.78 ± 0.12) SxL RIL ELISA-2008 SxL RIL SS-2008 25 30 20 20 15 RIL number 10 10 5 0 3 4 1 2 1 2 0 0.5 1.5 Virus concentration (ELISA values), A 405 nm Symptom severity (SS), score unit: scale 0-4

  4. G (43.0 ± 2.58) L (52.2 ± 0.10) S (43.7 ± 1.62) G (0.58 ± 0.07) L (0.61 ± 0.10) S (0.54 ± 0.06) S (5.41 ± 0.18) L (7.74 ± 0.11) G (2.17 ± 0.50) SxL RIL SPAD-2008 SxL RIL SR-2008 50 50 40 40 30 30 20 20 10 10 0.50 0.60 0.65 0.70 0.40 0.45 0.55 45 35 40 50 55 Leaf greenness (SPAD), SPAD units Spectral reflectance (NDVI), NDVI units SxL RIL GY-2008 30 20 10 8 7 9 4 6 2 3 5 Grain yield (GY), t ha-1

  5. G (3.81 ± 0.25) L (0.65 ± 0.35) S (2.88 ± 0.17) G (1.62 ± 0.08) L (0.17 ± 0.06) S (1.50 ± 0.15) SxL RIL ELISA-2009 SxL RIL SS-2009 25 30 20 20 15 RIL number 10 10 5 0 3 4 1 2 1 2 0 0.5 1.5 Virus concentration (ELISA values), A 405 nm Symptom severity (SS), score unit: scale 0-4

  6. G (30.2 ± 2.60) L (43.4 ± 2.00) S (32.6 ± 2.21) G (0.38 ± 0.06) L (0.59 ± 0.04) S (0.47 ± 0.03) G (3.67 ± 0.33) L (7.40 ± 1.46) S (5.00 ± 0.75) SxL RIL SPAD-2009 SxL RIL NDVI-2009 30 50 40 20 30 20 10 10 0.35 0.45 0.30 0.40 0.50 0.55 0.60 35 25 30 40 45 50 Leaf greenness (SPAD), SPAD units Spectral reflectance (NDVI), NDVI units SxL RIL GY-2009 30 20 10 8 7 9 4 6 2 3 5 Grain yield (GY), t ha-1

  7. Supplementary material Figure 2. Location of the QTLs controlling the SBCMV response in the Simeto × Levante recombinant inbred line (RIL) population, as evaluated in two field trials carried out in 2008 and in 2009. The location of the chromosome regions that were found significant on the basis of the composite interval mapping (CIM) is reported on the right side of the chromosome bars for each trait and for each scoring/sampling date. QTLs for the following traits are reported: visually estimated symptom severity (SS), virus concentration in the leaves (ELISA) and grain yield (GY). QTLs for GY were reported only when their positions overlapped with those of QTLs for direct SBCMV-response traits. The QTLs are indicated with acronyms including the trait acronym and the scoring/sampling date (indicated with a progressive number from 1 to 5, see materials and methods section for the corresponding dates). QTLs are indicated with vertical lines spanning the significance intervals (LOD = 2) and with triangles pointing to the QTL peak position. At each QTL region, the parent contributing the favorable allele has been reported within curly brackets (S = Simeto or L = Levante).

  8. Chr. 2A ELISA-2-09 GY-09 SS-2-08 ELISA-4-09 SS-3-09 L SS-2-09 SS-1-08 SS-4-09 SS-5-09 SS-3-08 SS-1-09

  9. Chr. 2B SS-3-08 GY-08 ELISA-2-08 L GY-09 SS-2-08 SS-1-08 SS-3-09 ELISA-4-09 SS-2-09 SS-4-09 SS-5-09 ELISA-3-08 ELISA-2-09 SS-1-09

  10. Chr. 3A SS-2-08 ELISA-4-09 SS-5-09 S SS-1-08 SS-1-09 SS-3-09 SS-3-08 ELISA-2-09 SS-2-09 GY-08 SS-4-09

  11. Chr. 5A Chr. 4B GY-08 ELISA-4-09 L ELISA-4-08 GY-08 SS-1-09 L SS-2-08

  12. Chr. 7A Chr. 6B L SS-3-09 SS-4-09 GY-08 S SS-3-08

  13. Chr. 7B GY-08 L ELISA-2-08

More Related