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M P N

M P N. PRESENTED BY: * NIATI NINGSIH * RATU TINTIN P * EMILIA WIDYASARI * IIN NARULITA F * FAIZAL ANDRI * LIA NOVA L. M O S T. P R O B A B L E. N U M B E R. INTRODUCTION. M P N. A method to count viable MO in a sample. ( Sutton, 2010 ). Commonly used in. Microbiological control.

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M P N

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  1. M P N PRESENTED BY: * NIATI NINGSIH * RATU TINTIN P * EMILIA WIDYASARI * IIN NARULITA F * FAIZAL ANDRI * LIA NOVA L M O S T P R O B A B L E N U M B E R

  2. INTRODUCTION M P N A method to count viable MO in a sample ( Sutton, 2010 ) Commonly used in Microbiological control Environmental monitoring ( USP, 2007 ) Pharmaceutical industry

  3. HISTORY 1968 De Mann 1957 Convidence interval table of MPN 1950 Woodward Cochran 1915 Neglect positive result on high dilution Strongest estimation of MPN Tabel Mc Crady Basic Statistic of MPN Method

  4. ASSUMPTION 1 MO distributed through the sample 2 MO separate individually not in chain

  5. ASSUMPTION 3 Media is suitable to growing MO 4 Just show amount of viable MO SIMULATIONS

  6. Prepare the sample and dilution series SAMPLE 1 1 ml 1 ml 1 ml 10-1 10-2 10-3 9 ml 9 ml 9 ml 1 ml 1 ml 1 ml Durham’s Tube

  7. Incubated 24 Hours 2

  8. Identify Positive Tube 3 Gas production Indicate positive result

  9. Count Positive Tube 4 3 2 1

  10. Convert to MPN Tabel 5

  11. Refference Cochran, W. 1950. Estimation of Bacterial Densities by Means of the Most Probable Number. Biometrics 6:105-116. Sutton, S. 2010. The Most Probable Number Method and Its Uses in Enumeration, Qualification, and Validation Journal of Validation Technology Vol. 16(3): 35-38. USP, Chapter <1116>. 2007. Microbiological Control and Monitoring Environments Used for the Manufacture of Healthcare Products. Pharm Forum 33(3).

  12. T H A N K O Y U

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