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Use of Biodiesel Waste Glycerol in the Bacterial Production of Polyhydroxyalkanoate (PHA) Biodegradable Plastic

Use of Biodiesel Waste Glycerol in the Bacterial Production of Polyhydroxyalkanoate (PHA) Biodegradable Plastic. Daniel Nicholson Graduate Student, Department of Paper & Bioprocess Engineering. ESF Biodiesel Production Program. +. Transesterification. 90% Biodiesel

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Use of Biodiesel Waste Glycerol in the Bacterial Production of Polyhydroxyalkanoate (PHA) Biodegradable Plastic

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  1. Use of Biodiesel Waste Glycerol in the Bacterial Production of Polyhydroxyalkanoate (PHA) Biodegradable Plastic Daniel Nicholson Graduate Student, Department of Paper & Bioprocess Engineering

  2. ESF Biodiesel Production Program + Transesterification 90% Biodiesel Fatty acid methyl ester 10% Glycerol Triglyceride Biodiesel is produced on the SUNY ESF campus from waste fryer oil from SU food services, for use in college vehicles and equipment . • The current US biodiesel production capacity is nearing 1 billion gal per year. • The co-product stream of waste glycerol could surpass 100 million galper year in the near future, and many possible uses for this essentially free raw material are under investigation.

  3. Polyhydroxyalkanoate (PHA) Chemistry R = CH3: Hydroxybutyrate (HB) R = CH2CH3: Hydroxyvalerate (HV) R = 3-6 Carbon side chain: Medium chain length (mcl) PHAs with more elastomeric properties. Increased side chain (R) length imparts flexibility to the polymer. Microbiologically produced poly-3-hydroxyalkanoate (PHA) polymers are a well known biodegradable material with physical characteristics comparable to some commercially available plastics like polypropylene and polyethylene.

  4. PHA Biodegradability Biodegradability studies have shown that many bacteria and fungi excrete PHA degrading enzymes in soil, sludge, fresh and sea water. These enzymes hydrolyze solid PHA into water-soluble oligomers and monomers, and use the resulting products as nutrients within their cells. Weeks in sewage sludge 0 2 8 4 6 Jendrossek, D. 2001. Adv. Biochem Eng Biotechnol. 71. 294-325

  5. PHA Production Life Cycle

  6. Bacterial PHA Production Burkholderia cepacia is a bacteria common in soil and water. This organism is being used in research at SUNY ESF, and has been shown to produce PHAs from different food sources. Consumes a number of foods, and stores extra in granules. PHA is the material stored to conserve energy and Carbon for future use. Benchtop bioreactors can be used to carefully control growth, and to observe product formation and food consumption.

  7. Biotechnology @ ESF For more information you can email Dr. Nomura ctnomura@esf.edu Lab webpage: http://www.esf.edu/chemistry/nomura/lab/ • Current projects • Metabolic and protein engineering for the production of biodegradable plastics • Microbial biodiesel production • Engineering of cellulase enzymes • Looking for graduate student applicants with strong backgrounds in: • Biochemistry • Molecular Biology • Microbiology • Synthetic organic chemistry

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