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EFFECT OF MICROGRAVITY ON THE ANTIBIOTIC RESISTANCE OF P. AREUGINOSA

EFFECT OF MICROGRAVITY ON THE ANTIBIOTIC RESISTANCE OF P. AREUGINOSA. Mitchell Hee , Jennifer Jiang, Jasmine Kuo , Annie Surman San Marino High School San Marino, California. Background. Astronaut Fred Haise infected by P. aeruginosa on Apollo 13 (1970)

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EFFECT OF MICROGRAVITY ON THE ANTIBIOTIC RESISTANCE OF P. AREUGINOSA

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  1. EFFECT OF MICROGRAVITY ON THE ANTIBIOTIC RESISTANCE OF P. AREUGINOSA Mitchell Hee, Jennifer Jiang, Jasmine Kuo, Annie Surman San Marino High School San Marino, California

  2. Background • Astronaut Fred Haise infected by P. aeruginosaon Apollo 13 (1970) • Bacteria contaminated water supply • Spaceflight is known to decrease human immune response • Affects T-Cell production

  3. Pseudomonas aeruginosa facultative anaerobe innate resistance to variety of antibiotics can easily develop new resistance often found in soil very few nutritional requirements affects those with compromised immune system or exposed tissue

  4. Bacteria Microgravity ?

  5. Materials • Type 3 FME • Main Volume: 5.50 ml of broth medium • 0.5% peptone • 0.3% chicken extract • 0.5% NaCl • 0.5% arabinose • Short Ampoule A: 1 mg freeze-dried P. aeruginosa • Short Ampoule B: 0.512 ml of 0.5 mg/ml L-Valine in distilled water A B

  6. Assembly procedures Ampoule B: L-Valine Ampoule A: P. aeruginosa Growth Medium

  7. Experiment procedures • Initiation; crack Ampoule A – A+1 • Termination; crack Ampoule B – A+3 • Upon return to earth: • Samples from flight and home combined with isoleucineto initiate growth • Plated on agar • Allowed to reproduce overnight at 37°C P. aeruginosa Isoleucine Antibiotic discs

  8. Zone of Inhibition (mm)

  9. Analysis/Conclusion • P. aeruginosais: • More resistant to streptomycin and gentamicin • Less resistant to ciprofloxacin • Remained resistant to ampicillin (ground and flight) • Inaccuraciesdue to: • A mishap in experiment procedures • Broken Teflon tube • Microgravity aids the growth of P. aeruginosa • Allows for suspension in growth medium

  10. Further steps • Measure minimum inhibitory concentration • Indicator to compare growth • Use of spectrophotometer • Change antibiotics to correct redundancy

  11. Acknowledgements Mr. Wyeth Collo Dr. Ali Khoshnan SSEP Nanoracks California Institute of Technology The Smithsonian Institution

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