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Fold activation

5. -. -. pG5Luc. -. -. 4. +. -. -. +. 3. Fold activation. +. +. 2. 1. 0. -. +. +. -. -. -. -. Gal4-MEF2C(2-473). -. -. -. +. -. -. -. Gal4-MEF2C(2-117). +. -. -. -. -. -. -. Gal4-MEF2C( 117-473 ). -. -. -. -. +. -. -. Gal4-MEF2C( 2-57 ). -. -.

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Fold activation

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  1. 5 - - pG5Luc - - 4 + - - + 3 Fold activation + + 2 1 0 - + + - - - - Gal4-MEF2C(2-473) - - - + - - - Gal4-MEF2C(2-117) + - - - - - - Gal4-MEF2C(117-473) - - - - + - - Gal4-MEF2C(2-57) - - - - - + - Gal4-MEF2C(2-57, 86-473) - - - - - - + Gal4-MEF2C(56-473) - + + + + + + FLAG-NFATc3 + CnA 5 pG5Luc 4 3 Fold activation 2 1 0 + + - Gal4-NFATc3(2-1110) - - + Gal4-NFATc3(2-314) - - - Gal4-NFATc3(314-732) - - - Gal4-NFATc3(732-1110) - + + MEF2C-FLAG Supplemental Fig 1 B A

  2. 40 35 30 1 58 87 473 25 TAD MADS MEF2 20 15 1 117 DE1 DE3 10 AdDNMEF2 myc LITR CMV GFP CMV 5 R24L 0 3xMEF2Luc MEF2A - - - - - - - - MEF2C1-117 - - - - - - - - MEF2C1-117R24L - - - - - - - - MEF2A1-131 + - + + + + + + + + + + + + + + + + + + + + Supplemental Fig 2 A B

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