1 / 8

1 2 3 4 5 6

Experiment A: Ergebnisse der X-gal-Topagar-Detektion der -Galaktosidase-Aktivität aller in der ersten Serie isolierten Mutanten. SD-TRP-URA. SD-TRP-URA. X-gal –LEU-TRP. Gr. 1. Gr. 2. Gr. 3. Gr. 4. Gr. 5. Gr. 6. Gr. 7. wt. 1 2 3 4 5 6. Experiment A:

nora
Download Presentation

1 2 3 4 5 6

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript


  1. Experiment A: Ergebnisse der X-gal-Topagar-Detektion der -Galaktosidase-Aktivität aller in der ersten Serie isolierten Mutanten SD-TRP-URA SD-TRP-URA X-gal –LEU-TRP Gr. 1 Gr. 2 Gr. 3 Gr. 4 Gr. 5 Gr. 6 Gr. 7 wt 1 2 3 4 5 6

  2. Experiment A: Ergebnisse der X-gal-Topagar-Detektion der -Galaktosidase-Aktivität aller in der zweiten Serie isolierten Mutanten 1 2 3 4 5 6 7 8 9 10 11 12 Gr. 1 Gr. 2 Gr. 3 Gr. 4 X-gal –LEU-TRP Gr. 5 Gr. 6 Gr. 7 13-1 13-2 13-3 13-4 13-5 13-6 13-7 wt wt wt wt wt Die Auswertung der Kreuzung dieser Serie dauert noch an.

  3. Experiment C: Cycloheximide Chase Analyse von DHFR-mutD-HA CDC11 mutD-ha2 CDC11 mutD-ha2 CDC11 mutD-ha2

  4. Experiment C: Quantifizierung der Western Blot Signale von DHFR-mutD-HA normalisiert gegen die CDC11-Signale CDC11 DHFRmutD-HA

  5. PGK Pup1 Experiment C: Quantifizierung der Western Blot Signale von PUP1/2 0 1 2 ON ON = over night

  6. mutant DHFR proteins fused to GFP form microscopically detectable aggregates DHFRwt-GFP DHFRmutC-GFP no aggregates cells often containd single aggregates

  7. GBD-ODC GBD GAD-Oaz1 GAD-ODC GAD SD ohne Leu, Trp, His SD ohne Leu, Trp, Ade Two hybrid assay Versuch E SD ohne Leu, Trp Kreuzungen nach Replika

  8. 1 2 3 4 5 6 7 8 9 10 anti-alpha7 1 JD47-13C ("Wildtyp") 2 ump1∆ 3 beta1 4 beta2 5 beta3 6 beta4 7 beta5 8 beta6 9 beta7 10 UMP1-HA Versuch C 1 2 3 4 5 6 7 8 9 10 anti-beta2 1 2 3 4 5 6 7 8 9 10 anti-Rpt5

More Related