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P450 (heme)

NAD(P)H. NAD(P) +. Fe-S. P450 (heme). FAD. NADP +. NADPH. FAD-FMN. HOOC. NH 2. a class I (bacteria; mitochondria). b class II (ER in eukaryotes). NADPH. NADP +. P450 (heme). FAD-FMN. ENDOPLASMIC RETICULUM. ER membrane. c P450RhF ( Rhodococcus sp.).

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P450 (heme)

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  1. NAD(P)H NAD(P)+ Fe-S P450 (heme) FAD NADP+ NADPH FAD-FMN HOOC NH2 aclass I (bacteria; mitochondria) bclass II (ER in eukaryotes) NADPH NADP+ P450 (heme) FAD-FMN ENDOPLASMIC RETICULUM ER membrane cP450RhF(Rhodococcus sp.) dP450BM3(Bacillus megaterium) NAD(P)H NAD(P)+ FMN P450 (heme) NH2 P450 (heme) Fe-S COOH Additionalfile 1 (Figure 1)

  2. a heterologous P450 (class I) gene SacI EcoRI NdeI FMN Fe-S T7promoter the RhFRed gene Linker sequence from P450RhF Apr pRED lacI (6.4 kb) ori pET21a Additionalfile 1 (Figure 2)

  3. Additionalfile 1 (Figure 3)

  4. Additionalfile 1 (Figure 4)

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