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Catalyst – What steps could you take to physically see your dna ?. Thursday, 10.30.2008. Tomorrow – Cheek DNA Lab. Purpose: To Isolate your own DNA from your cheek cells. Notes: BRING YOUR TOOTHBRUSH! DO NOT BRUSH YOUR TEETH UNTIL CLASS – I will bring tooth paste (and you can too).
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Catalyst – What steps could you take to physically see your dna? Thursday, 10.30.2008
Tomorrow – Cheek DNA Lab • Purpose: To Isolate your own DNA from your cheek cells. • Notes: BRING YOUR TOOTHBRUSH! DO NOT BRUSH YOUR TEETH UNTIL CLASS – I will bring tooth paste (and you can too)
Materials • 0.9% Salt Water Solution • 25% Soap Solution • 95% Ethyl Alcohol (EtOH) • Cheek Cells • Various Cups • Stirring Rods
Create a Flow Chart • 1. Swish 10mL of salt water in your mouth for about 30s-1min. • 2. Spit water into small cup. • 3. Pour spit water into tube containing 5mL of soap solution. • 4. Gently mix this solution for about 2-3 minutes. • 5. Pour 10-15mL of ice cold EtOH down the side of the tube without mixing the layers. • 6. Gently rotate the tubes.