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Biochemistry 455 Fall 2009

Instructor: Gail Grabner (ggrabner@illinois.edu) Topics: Protein purification, Dipeptide sequence analysis, Molecular cloning, ELISA, Lipid analysis. Student comments: “I took a lot out of this class; almost nothing was repetitive of anything I had learned in any other lab.”

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Biochemistry 455 Fall 2009

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  1. Instructor: Gail Grabner (ggrabner@illinois.edu) Topics: Protein purification, Dipeptide sequence analysis, Molecular cloning, ELISA, Lipid analysis Student comments: “I took a lot out of this class; almost nothing was repetitive of anything I had learned in any other lab.” “I feel that my lab skills improved after taking BIOC 455. I’ve gained more experience and learned a lot of techniques and assays.” “The techniques used here are genuinely applicable, particularly for students already doing research & considering graduate school.” “Things I learned in BIOC 455 will most definitely help me when I enter the workforce.” Biochemistry 455 Fall 2009 Techniques in Biochemistry & Biotechnology

  2. Techniques in Biochemistry & Biotechnology : BIOC 455 CREDITS: 4 hours WHEN: M, W Lecture: 1:00-1:50; Lab: 2:00-4:50 WHERE: Lab in 218 Noyes Lab INSTRUCTOR: Gail K. Grabner CONTACT: ggrabner@illinois.edu

  3. BIOC 455: Course Content • Dipeptide analysis (2 lab periods) • Determination of the amino acid sequence of a dipeptide • Photometry (1 lab period) • Colorimetric quantitation of total protein concentration.   • Study of the properties of β-galactosidase (4 lab periods) • Kinetic assays of β-galactosidase • Study of carbohydrates (1 lab period) • Enzymatic and chemical determination of carbohydrate unknowns • Purification of Glutamate Oxaloacetate Transaminase from pig heart muscle (5 lab periods) • Three step purification of an active enzyme from a natural source.  

  4. BIOC 455: Course Content • Study of the interaction of RNA polymerase subunits. (1 lab period) • Affinity pull-down of separately expressed RNA polymerase subunits from Methanococcus jannaschii.  • Study of the properties of aspartate transcarbamylase (1 lab period) • Kinetic assays of the allosteric enzyme ATCase • Use of the ELISA in immunology (3 lab periods) • Determination of the titers of purified sera, unpurified sera, and monoclonal ascites.  • Characterization of Erythrocyte Membranes (2 lab periods) • Extraction of protein and lipid fractions of pig red blood cells.  • In vitro transcription/translation (2 lab periods) • Coupled system to produce isotopically labeled protein product

  5. BIOC 455: Student Comments

  6. BIOC 455: Student Comments

  7. BIOC 455: Student Comments

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