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Soybean Suspensions

Soybean Suspensions. James D. Wilson STEP Science Camp May 21 to June 1, 2001 Advisor: Antoine Al-Achi, Ph.D. Introduction. Soybean has been cultivated in China for thousands of years. Currently, the United States is the main producer of Soybean worldwide.

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Soybean Suspensions

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  1. Soybean Suspensions James D. Wilson STEP Science Camp May 21 to June 1, 2001 Advisor: Antoine Al-Achi, Ph.D. James D. Wilson/Antoine Al-Achi

  2. Introduction • Soybean has been cultivated in China for thousands of years. • Currently, the United States is the main producer of Soybean worldwide. • Soybean is commonly used in food industry, especially in Asia. • Soybean is rich in proteins and oil. It contains all the essential proteins needed for life. James D. Wilson/Antoine Al-Achi

  3. Objectives • To prepare suspensions containing soybean powder with different suspending agents. • To test the ability of soybean powder to act as a suspending agent itself. James D. Wilson/Antoine Al-Achi

  4. Soybean Formulas James D. Wilson/Antoine Al-Achi

  5. Phosphate Buffer Solution Potassium Phosphate, Monobasic (0.2 M) 250 ml Sodium Hydroxide (0.2 M) 145.5 ml Purified Water qs. ad. 1 liter pH = 7.00 James D. Wilson/Antoine Al-Achi

  6. Formula 1: Soybean Control Soybean 12 g Glycerin 3.2 ml Orange Oil 2 Drops Syrup, NF 2 ml Buffer Solution (pH = 7.0) qs 60 ml James D. Wilson/Antoine Al-Achi

  7. Formula 2: Soybean with Tween 20 Soybean 12 g Glycerin 3.2 ml Orange Oil 2 Drops Syrup, NF 2 ml Tween 20 5 ml Buffer Solution (pH = 7.0) qs 60 ml James D. Wilson/Antoine Al-Achi

  8. Formula 3: Soybean with Acacia and Gum Tragacanth Soybean 12 g Glycerin 3.2 ml Orange Oil 2 Drops Syrup, NF 2 ml Acacia 1.5 g Gum Tragacanth 0.3 g Buffer Solution (pH = 7.0) qs 60 ml James D. Wilson/Antoine Al-Achi

  9. Formula 4: Soybean with Bentonite Magma Soybean 12 g Glycerin 3.2 ml Orange Oil 2 Drops Syrup, NF 2 ml Bentonite Magma 12 ml Buffer Solution (pH = 7.0) qs 60 ml James D. Wilson/Antoine Al-Achi

  10. Results James D. Wilson/Antoine Al-Achi

  11. Control By Time (hrs) Control By Time (hrs) James D. Wilson/Antoine Al-Achi

  12. Tween By Time (hrs) Tween By Time (hrs) James D. Wilson/Antoine Al-Achi

  13. Acacia/Tragacanth By Time (hrs) Acacia/Tragacanth By Time (hrs) James D. Wilson/Antoine Al-Achi

  14. Bentonite Magma By Time (hrs) Bentonite By Time (hrs) James D. Wilson/Antoine Al-Achi

  15. Sedimentation Volume (F) Suspension TypeF Value† Control 0.74 ± 0.056 Tween 20 0.68 ± 0.094 Acacia & Gum Tragacanth 0.83 ± 0.034 Bentonite Magma 0.87 ± 0.024 † Mean ± S.D. (n = 6) James D. Wilson/Antoine Al-Achi

  16. Degree of Flocculation () Suspension Type Value† Control 1.00 Tween 20 0.98 ± 0.14 Acacia & Gum Tragacanth 1.20 ± 0.05 Bentonite Magma 1.26 ± 0.03 James D. Wilson/Antoine Al-Achi

  17. † Mean ± S.D. (n = 6) James D. Wilson/Antoine Al-Achi

  18. Calamine Lotion with Soybean James D. Wilson/Antoine Al-Achi

  19. Formula 1: Calamine Lotion Control Calamine 4.8 g Zinc Oxide 4.8 g Glycerin 1.2 ml Lime Water qs ad. 60 ml James D. Wilson/Antoine Al-Achi

  20. Formula 2: Calamine Lotion with Bentonite Magma Calamine 4.8 g Zinc Oxide 4.8 g Glycerin 1.2 ml Bentonite Magma 24 ml Lime Water qs ad. 60 ml James D. Wilson/Antoine Al-Achi

  21. Formula 3: Calamine Lotion with Soybean Calamine 4.8 g Zinc Oxide 4.8 g Glycerin 1.2 ml Soybean Suspension (5%) 24 ml Lime Water qs ad. 60 ml James D. Wilson/Antoine Al-Achi

  22. Results James D. Wilson/Antoine Al-Achi

  23. Calamine Lotion Control by Time (hrs) Control By Time (hrs) James D. Wilson/Antoine Al-Achi

  24. Calamine Lotion with Bentonite Magma by Time (hrs) Bentonite By Time (hrs) James D. Wilson/Antoine Al-Achi

  25. Calamine Lotion with Soybean by Time (hrs) Soybean By Time (hrs) James D. Wilson/Antoine Al-Achi

  26. Sedimentation Volume (F) Suspension TypeF Value† Control 0.39 ± 0.04 Soybean 0.23 ± 0.02 Bentonite Magma 0.69 ± 0.05 † Mean ± S.D. (n = 6) James D. Wilson/Antoine Al-Achi

  27. Degree of Flocculation () Suspension Type Value† Control 1.00 Soybean 0.63 ± 0.03 Bentonite Magma 1.84 ± 0.10 † Mean ± S.D. (n = 6) James D. Wilson/Antoine Al-Achi

  28. Conclusion • Soybean suspensions made with different suspending agents showed that bentonite magma produced the best dispersion as compared to the other formulations. This was evident by the settling rate, sedimentation volume (F), and degree of flocculation (). James D. Wilson/Antoine Al-Achi

  29. Conclusion (Continued) • When soybean dispersion (5%) was compared to bentonite magma in the preparation of calamine lotion, bentonite magma produced a much better dispersion than that with soybean. This conclusion was reached based on comparing the settling rate, sedimentation volume, and degree of flocculation of suspensions made by these two agents. James D. Wilson/Antoine Al-Achi

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